论文部分内容阅读
目的筛选出愈肠宁方(黄芪多糖、黄芪皂苷、苦参碱、氧化苦参碱组成)用于治疗溃疡性结肠炎(ulcerative colitis,UC)的最佳给药方式,为其制剂设计提供依据。方法采用三硝基苯磺酸(TNBS)诱导大鼠UC模型,实验分为阳性组、正常组、模型组、给药组等6组,其中给药组按给药方式分为灌胃组(Ⅰ)、灌肠组(Ⅱ)、黄芪组分灌胃+苦参组分灌肠组(Ⅲ)3组,分别观察治疗后大鼠结肠黏膜组织形态学变化,测定计算结肠肠重系数、血清中肿瘤坏死因子α(TNF-α)、结肠黏膜组织中髓过氧化物酶(MPO)的水平。结果与模型组比较,Ⅱ、Ⅲ组均能明显改善结肠黏膜组织损伤、降低肠重系数(P<0.05,P<0.01),并显著降低TNF-α及MPO水平(P<0.01),而Ⅰ组仅能降低TNF-α水平(P<0.01)。结论愈肠宁方对该模型有较好的疗效,3种给药方式中以Ⅲ组效果最佳,为该方的制剂设计提供参考。
Objective To screen out the best administration mode of Yu-Chang-ning Fang (astragalus polysaccharide, astragalus saponin, matrine and oxymatrine) for the treatment of ulcerative colitis (UC) . Methods The rat model of UC was induced by trinitrobenzene sulfonic acid (TNBS). The experiment was divided into 6 groups: positive group, normal group, model group and administration group. The administration group was divided into gavage group (Ⅱ), Astragalus mongholicus group and enema group (Ⅲ) of Sophora flavescens group. The histomorphological changes of colonic mucosa in rats were observed and the colonic intestinal weight coefficient, the serum tumor Necrosis factor alpha (TNF-alpha), and myeloperoxidase (MPO) levels in colonic mucosa. Results Compared with the model group, the injury of colonic mucosa was significantly reduced in group Ⅱ and Ⅲ (P <0.05, P <0.01), and the levels of TNF-α and MPO were significantly decreased (P <0.01) Group can only reduce the level of TNF-α (P <0.01). Conclusion Yu-Chang-ning Fang has good curative effect on this model. The three kinds of administration modes are the best in group Ⅲ, which provide a reference for formulation design of the prescription.