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测定结果,在减数分裂期三系花药内游离脯氨酸含量都很少,相差无几,而到了单核期三系花药内游离脯氨酸含量差异较大,其含量保持系是不育系的13.5倍,恢复系是不育系的11倍。从减数分裂期进展到单核期花药内游离脯氨酸的含量,不育系减少了50％,而保持系和恢复系分别增加了12.5倍和6.33倍,此表明花药内脯氨酸是在减数分裂之后从四分体期开始随着花粉粒的充实、长大而迅速增加积累的。脯氨酸能为花粉的萌发和花粉管伸长提供氮源和能源,在花粉萌发过程中脯氨酸被直接用于蛋白质合成,而且脯氨酸很容易转化为同族的其它氨基酸,氨基酸又是合成蛋白质的原料。所以缺少脯氨酸必引起蛋白质合成受阻,从而进一步影响花粉粒的成长发育等生理活动,并影响其育性。因此,我们认为,高粱花药败育是在减数分裂后花粉形成过程中发生异常而形成了干瘪无花粉粒的花药导致败育的。不育系中的脯氨酸含量的减少也是在此时期发生的,并且花药的败育和脯氨酸含量的减少是同步进行的。因此,脯氨酸的含量与花药的败育有着密切的关系。
The results showed that there were very few free prolines in the three-line anther at the meiosis stage, but the content of free proline in the three-line anther at the mononuclear stage was quite different. 13.5 times, restorer lines are 11 times of CMS. From the stage of meiosis to the content of free proline in mononuclear anthers, the male sterile lines decreased by 50%, while the maintainer lines and restorers increased by 12.5 times and 6.33 times, respectively, indicating that proline in the anthers is After meiosis from the quaternary period with the pollen grains to enrich, grow and rapidly increase the accumulation. Proline can provide nitrogen source and energy for pollen germination and pollen tube elongation. Proline is used for protein synthesis directly during pollen germination, and proline can easily be transformed into other amino acids in the same family. Amino acids are Synthetic protein raw materials. Therefore, the lack of proline will cause protein synthesis blocked, thereby further affecting pollen grain growth and development and other physiological activities, and affect their fertility. Therefore, we think that the anther abortion of sorghum is aborted after the anthesis of pollen during meiosis, resulting in the formation of wilted anther. The decrease in proline content in CMS lines also occurred during this period, and abortion of anther and reduction of proline content were synchronized. Therefore, the content of proline and anther abortion have a close relationship.