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用地高辛标记的ＨＢＶ基因组探针检测了６６例肝细胞性肝癌（ＨＣＣ）中ＨＢＶ的存在状态，从中筛选出３２例仅有ＨＢＶＤＮＡ整合的ＨＣＣ作为研究对象，进一步检测了ＨＢＶＸ基因、Ｓ基因、Ｐｒｅ－Ｓ、Ｃ基因ＤＮＡ片段的整合情况，并探讨了ＨＢＶ４种基因亚片段整合与ｒａｓ、ｍｙｃ癌基因表达的关系。结果显示，ＨＢＶＸ、Ｓ、Ｐｒｅ－Ｓ和Ｃ基因片段的整合率分别为８７．５％（２８／３２）、６２．５％（２０／３２）、６２．５％（２０／３２）和２５．０％（８／３２）。ｐ２１ｒａｓ、ｐ６２ｍｙｃ在ＨＣＣ中的表达率分别为５０．０％（２０／４０）、８１．２％（２６／３２）。通过对ＨＣＣ中４种ＨＢＶ基因片段整合与ｐ２１ｒａｓ、ｐ６２ｍｙｃ表达之间关系的研究，显示ｐ６２ｍｙｃ的表达与ＨＢＶＸ、Ｐｒｅ－Ｓ基因整合关系密切，ｐ２１ｒａｓ的表达则主要与ＨＢＶＸ基因整合有关（Ｐ＜０．０５）。提示ＨＢＶＸ、Ｐｒｅ－ＳＤＮＡ片段在宿主细胞染色体上的整合可能直接或通过其蛋白启动ｍｙｃ和（或）ｒａｓ癌基因的表达。
The presence of HBV in 66 cases of hepatocellular carcinoma (HCC) was detected with a digoxigenin-labeled HBV genome probe, and 32 HCCs with only HBV DNA integration were screened out for further detection of HBVX gene, S gene, The integration of Pre-S and C gene DNA fragments, and the relationship between the integration of HBV four sub-fragments and the expression of ras and myc oncogenes. The results showed that the integration rates of HBVX, S, Pre-S, and C gene fragments were 87.5% (28/32), 62.5% (20/32), 62.5% (20/32), and 25, respectively. .0% (8/32). The expression rates of p21ras and p62myc in HCC were 50.0% (20/40) and 81.2% (26/32), respectively. The study of the relationship between the integration of four HBV gene fragments and the expression of p21ras and p62myc in HCC showed that the expression of p62myc is closely related to the integration of HBVX and Pre-S genes, and the expression of p21ras is mainly related to the integration of HBVX genes (P<0). .05). It suggests that the integration of HBVX and Pre-S DNA fragments on the chromosome of host cells may directly or through the expression of myc and/or ras oncogenes.