论文部分内容阅读
目的 :观察海水浸泡对火器伤伤口组织脂质过氧化反应的影响。方法 :以滑膛枪发射质量 2 5 0mg钢珠 ,致伤兔后肢 ,伤后将致伤兔随机分为两组 :一组为海水浸泡组 (SIG) ,将致伤兔浸泡于粗制海盐配制的人造海水中 30min ;另一组为单纯致伤组 (SWG) ,伤后不浸泡海水。伤后 3、6、12、2 4h手术取距伤道边缘 0 5cm(A区 )、1 5cm(B区 )和 2 5cm(C区 )处肌组织 ,测定ATP、丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活力。取伤前骨骼肌组织作为对照。结果 :伤后SIG兔伤肢骨骼肌MDA含量明显高于对照 ,伤后 3、6h升高 ,12h略降 ,2 4h有再次升高的趋势。SWG的MDA含量变化与SIG相似 ,但升幅低于SIG ;2组SOD活力和ATP含量的改变与MDA含量变化相似。结论 :海水浸泡的火器伤伤口脂质过氧化反应增强 ,从而加重了肢体火器伤的过氧化反应
Objective: To observe the effects of seawater immersion on lipid peroxidation in wounds of firearm. Methods: The muskets with mass of 250 mg were delivered by a muslin gun, the hind limbs of the rabbits were harmed, and the injured rabbits were randomly divided into two groups: one was a seawater immersion group (SIG), the injured rabbit was soaked in crude sea salt Artificial seawater 30min; the other group was a simple injury group (SWG), after the injury is not soaked seawater. At 3, 6, 12, and 24 hours after injury, the tissues from 0-5cm (area A), 15cm (area B) and 25cm (area C) of the wound were removed and the contents of ATP and malondialdehyde And superoxide dismutase (SOD) activity. Pre-injury skeletal muscle tissue as a control. Results: The content of MDA in skeletal muscle of injured limbs of SIG rabbits was significantly higher than that of the control group at 3 and 6 hours after injury, slightly decreased at 12 hours and increased again at 24 hours. The content of MDA in SWG was similar to SIG, but the increase was lower than that in SIG. The change of SOD activity and ATP content in SWG was similar to that of MDA. CONCLUSIONS: The lipid peroxidation in wounds exposed to seawater immersion is enhanced, which aggravates the peroxidation of limb firearm injuries