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目的 检测细胞核 DNA含量在大肠癌、大肠腺瘤及正常肠粘膜中的表达 ,探讨其在大肠癌恶性转化中的意义。 方法 应用 Feulgen染色及图像分析技术分别对 5 3例结肠癌及直肠癌、30例大肠腺瘤、10例正常肠粘膜进行 DNA含量分析。 结果 正常肠粘膜、腺瘤、腺癌三组 DNA含量差别有统计学意义 (P<0 .0 5 )。将检测结果行正常组织与癌组织、腺瘤与腺癌、腺瘤与正常组织之间做两两比较 ,除腺瘤与正常组织之间外 ,其他各组都有显著性差别。 结论 DNA含量测定对早期发现大肠腺癌有重要意义 ,为判断大肠疾病的性质提供细胞生物学依据。
Objective To detect the expression of nuclear DNA in colorectal cancer, colorectal adenoma and normal intestinal mucosa and to explore its significance in the malignant transformation of colorectal cancer. Methods The DNA content of 53 cases of colon and rectal cancer, 30 cases of colorectal adenoma and 10 cases of normal intestinal mucosa were analyzed by Feulgen staining and image analysis respectively. Results There was a significant difference in DNA content between normal mucosa, adenoma and adenocarcinoma (P <0.05). The test results line normal tissue and cancer, adenoma and adenocarcinoma, adenoma and normal tissue between the pairwise comparison, in addition to between the adenoma and normal tissue, the other groups have significant differences. Conclusion The determination of DNA content is of great significance for the early detection of colorectal adenocarcinoma and cell biology for the judgment of the nature of colorectal disease.