论文部分内容阅读
目的研究孕期流感病毒感染对后代小鼠肺组织形态和肺表面活性蛋白(surfactant protein,SP)-A、-D表达的影响,探讨影响机制。方法用非致死量甲型H1N1流感病毒A/四川/SWL1/2009 H1N1稀释液滴鼻感染孕15 d Balb/c孕鼠,在孕19 d随机选取孕鼠剖宫取其胎盘组织,并收集羊水标本;取胎鼠肺组织。待余下孕鼠自然分娩后,取P0,P4,P7,P14 d小鼠肺组织。观察后代小鼠肺组织病理学改变;检测其肺组织SP-A、-D的表达水平。检测胎盘、胎鼠肺组织流感病毒基因;检测羊水白介素-6(interleukin-6,IL-6),肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平。结果与对照组相比,感染组后代小鼠肺泡发育程度较低,肺泡隔厚度明显增加。SP-A的表达量从P4 d开始显著降低;SP-D的表达量从P7 d开始显著升高。未检测到胎盘或胎鼠肺组织流感病毒基因;羊水中IL-6,TNF-α水平明显升高。结论孕期流感病毒感染可能通过造成宫炎症环境间接改变后代肺组织形态和SP-A,-D的表达模式。
Objective To study the influence of influenza virus infection during pregnancy on the lung tissue morphology and the expression of surfactant protein (SP) -A and -D in offspring mice and to explore the possible mechanism. Methods Balb / c pregnant mice were intranasally infected with non-lethal A / H1N1 influenza A / Sichuan / SWL1 / 2009 H1N1 dilutions for 15 days. Pregnant cesarean sections were randomly selected at 19 days of gestation for placental tissue collection and amniotic fluid Specimens; take fetal rat lung tissue. After the remaining pregnant mice to be natural childbirth, take P0, P4, P7, P14 d mice lung tissue. Observe the pathological changes of lung tissue of the offspring mouse; detect the expression of SP-A and -D in the lung tissue. The placenta and fetal rat lung tissue were tested for influenza virus. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected. Results Compared with the control group, the progeny of the infected mice showed lower alveolar development and a significant increase in the alveolar septum thickness. The expression of SP-A decreased significantly from P4 d, while the expression of SP-D increased significantly from P7 d. No placenta or fetal rat lung influenza virus gene was detected; amniotic fluid IL-6, TNF-α levels were significantly higher. Conclusions Influenza virus infection during pregnancy may indirectly change the lung tissue morphology and SP-A and -D expression patterns of offspring through the intrauterine inflammatory environment.