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目的:检测miR-141-3p在胃癌组织及患者血浆中的表达水平,探讨其表达水平与患者病理特征和预后的关系。方法:收集河北医科大学第四医院普外科2012年5月至2013年1月胃癌根治性手术切除的组织标本及术前外周静脉血标本44例,每例标本采集肿瘤组织(非坏死部分)和配对的癌旁组织。采用实时荧光定量PCR法检测miR-141-3p在胃癌组织、癌旁组织、血浆及健康志愿者血浆(25例)的表达情况,分析miR-141-3p的表达水平与胃癌患者DFS及临床病理特征的关系。结果:miR-141-3p在胃癌组织中的表达明显降低,在血浆中的表达水平明显升高(均P<0.01)。在胃癌组织中miR-141-3p高表达组DFS明显高于低表达组(21.8 vs 10.3个月,P<0.01),且miR-141-3p在癌组织中的表达水平与患者DFS呈正相关(P<0.01)。在血浆中miR-141-3p的高表达组DFS明显低于低表达组(9.1 vs 21.0个月,P<0.01),且miR-141-3p血浆中的表达水平与患者DFS呈负相关(P<0.01)。miR-141-3p在胃癌患者血浆中表达水平与肿瘤TNM分期、淋巴结转移及脉管瘤栓相关(P<0.01)。结论:miR-141-3p在胃癌组织中表达显著降低且与患者预后呈正相关,其在患者血浆中表达显著升高且与患者预后呈负相关,表明miR-141-3p可作为胃癌早诊早治及患者临床预后判定的潜在标志物。
OBJECTIVE: To detect the expression of miR-141-3p in the plasma of patients with gastric cancer and its relationship with the pathological features and prognosis. Methods: Totally 44 cases of radical resection of gastric cancer and preoperative peripheral venous blood samples were collected from May 2012 to January 2013 in the Department of General Surgery, the Fourth Hospital of Hebei Medical University. Tumor samples (non-necrotic parts) and Paired paracancer tissue. The expression of miR-141-3p in gastric cancer tissue, paracancerous tissues, plasma and healthy volunteers plasma (25 cases) was detected by real-time fluorescence quantitative PCR. The expression of miR-141-3p in gastric cancer patients with DFS and clinical pathology Characteristics of the relationship. Results: The expression of miR-141-3p was significantly decreased in gastric cancer tissues and significantly increased in plasma (all P <0.01). The DFS of miR-141-3p high expression group was significantly higher than that of low expression group (21.8 vs 10.3 months, P <0.01) in gastric cancer tissues, and the expression of miR-141-3p in cancer tissues was positively correlated with DFS P <0.01). The DFS of miR-141-3p overexpression in plasma was significantly lower than that in the low expression group (9.1 vs 21.0 months, P <0.01), and the plasma levels of miR-141-3p were negatively correlated with DFS <0.01). The expression of miR-141-3p in plasma of gastric cancer patients was correlated with TNM staging, lymph node metastasis and tumor thrombus (P <0.01). Conclusion: The expression of miR-141-3p in gastric cancer was significantly decreased and positively correlated with the prognosis of patients. The expression of miR-141-3p in the plasma was significantly increased and negatively correlated with the prognosis of patients, indicating that miR-141-3p could be used as a early diagnosis of gastric cancer Governance and prognosis of patients with potential markers.