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目的 从mRNA水平探讨白细胞介素2、6(IL-2、IL -6)在慢性乙型肝炎发病机制中的作用。方法 收集慢性迁延性乙型肝炎 (CPH)、慢性活动性乙型肝炎 (CAH)患者以及接种乙肝疫苗的青少年和正常人的外周血单个核细胞 (PBMC) ,以HBsAg、HBeAg以及植物血凝素 (PHA)刺激后 ,提取其总RNA ,采用半定量核酸杂交技术 ,检测经刺激后的PBMC表达的IL-2mRNA和IL-6mRNA水平。结果 CPH、CAH患者的PBMC经PHA刺激后 ,表达的IL-2、IL-6mRNA水平与接种乙肝疫苗者和正常人无明显差异 (均P>0.05) ) ;而经HBsAg及HBeAg刺激后 ,IL-2mRNA水平与接种乙肝疫苗者和正常人相比明显低下 (均P<0.01) ;经HBeAg刺激后 ,IL-6mRNA水平明显高于接种乙肝疫苗者和正常人 (P<0.01)。结论 慢性乙型肝炎患者的IL-2mRNA的水平下降 ,HBeAg诱生IL-6mRNA的水平明显高于HBsAg,且与血清谷丙转氨酶水平的升高相一致
Objective To investigate the role of interleukin-2 (IL-2, IL-6) in the pathogenesis of chronic hepatitis B from mRNA level. Methods Peripheral blood mononuclear cells (PBMCs) from chronic persistent hepatitis B (CPH) and chronic active hepatitis B (CAH) patients as well as from adolescents and healthy subjects who were given hepatitis B vaccine were collected. The HBsAg, HBeAg and phytohemagglutinin (PHA), total RNA was extracted and the levels of IL-2 mRNA and IL-6 mRNA were detected by semi-quantitative nucleic acid hybridization. Results The levels of IL-2 and IL-6 mRNA in PBMCs of CPH and CAH patients after PHA stimulation were not significantly different from those of HBsAg and HBeAg vaccines (P> 0.05) -2 mRNA levels were significantly lower than those who received hepatitis B vaccine (P <0.01). After stimulation with HBeAg, the level of IL-6 mRNA was significantly higher than those vaccinated with hepatitis B vaccine and normal controls (P <0.01). Conclusion The level of IL-2 mRNA in patients with chronic hepatitis B is decreased, the level of IL-6 mRNA induced by HBeAg is significantly higher than that of HBsAg, and it is consistent with the increase of serum alanine aminotransferase