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抗坏血酸是调整植物细胞氧化还原过程的重要物质。随着果实的成熟,抗坏血酸含量逐渐增高。因此,利用抗坏血酸的含量可作为判定成熟和最佳采收期的一个指标。以往测定抗坏血酸常用2,6——二氯吲哚酚钠法。山葡萄中的抗坏血酸含量测定尚未见报道。由于2,6——二氯吲哚酚钠法滴定终点的颜色与山葡萄果汁颜色类似,使终点不易判定,故本实验采用紫外分光光度计,用铝酸铵显色,在λmax824nm处,以标准品为对照。对开始成熟的不同时期所采收的左山一、74003、双庆三个品种(系)鲜样进行测
Ascorbic acid is an important substance that regulates the redox process of plant cells. As the fruit matures, the content of ascorbic acid gradually increases. Therefore, the use of ascorbic acid content can be used as an indicator of maturity and optimal harvest time. In the past, ascorbic acid was commonly used 2,6-dichlorophenolin sodium method. Determination of ascorbic acid in grape vines has not been reported. Since the color of the end point of 2,6-dichlorophenolindophenol sodium titration is similar to the color of grape juice in mountainous region, the end point is not easy to be determined. Therefore, this experiment uses an ultraviolet spectrophotometer to develop color with ammonium aluminate, and the standard is at λmax824nm. The product is a control. To test the fresh samples of the three varieties (lines) of Zuoshan I, 74003 and Shuangqing collected at different stages of maturation