论文部分内容阅读
目的评价异氟烷3种干预方式的脑保护效能,为临床提供脑缺血保护依据。方法雄性SD大鼠随机分为假手术组、对照组、预处理组、保护组、复苏组,后4组按缺血时间再分为缺血10min、15min及20min3个亚组。建立清醒全脑缺血模型。观察清醒、缺血及再灌注后海马谷氨酸递质浓度及BIS改变,记录翻正反射恢复的时间及运动功能评分。记数海马CA1区锥体细胞数和TUNEL阳性细胞的百分率。结果保护组在缺血15min翻正反射恢复时间短于预处理组(P<0.05)。保护组缺血10min和15min期间谷氨酸浓度低于预处理组和复苏组(P<0.01)。全脑缺血10min及15min,保护组海马CA1区神经细胞计数高于预处理组及复苏组(P<0.05)。缺血10min及15min保护组的细胞凋亡率明显低于预处理组和复苏组(P<0.05)。结论异氟烷麻醉下的脑保护效应要好于预处理及复苏;异氟烷预处理与复苏的脑保护效应相同。
Objective To evaluate the protective efficacy of 3 kinds of isoflurane on cerebral protection and provide the basis for clinical protection of cerebral ischemia. Methods Male SD rats were randomly divided into sham operation group, control group, preconditioning group, protective group, resuscitation group and the last 4 groups according to the ischemic time and then subdivided into 3 subgroups: ischemia 10min, 15min and 20min. Establishment of conscious whole brain ischemia model. The changes of glutamate neurotransmitter concentration and BIS in the hippocampus after awake, ischemia and reperfusion were observed. The recovery time and motor function score of the reflex reflex were recorded. The number of pyramidal neurons and TUNEL positive cells in hippocampal CA1 area were counted. Results In the protective group, the recovery time of righting reflex was shorter than that of the pretreatment group (P <0.05) 15min after ischemia. The concentration of glutamate in the protective group was lower than that of the preconditioning group and the resuscitation group at 10min and 15min (P <0.01). At 10 minutes and 15 minutes after global cerebral ischemia, the number of neurons in hippocampal CA1 area of hippocampus in protection group was higher than those in preconditioning and resuscitation groups (P <0.05). The apoptosis rate of the protective group at 10 min and 15 min after ischemia was significantly lower than that of the pretreatment group and the resuscitation group (P <0.05). Conclusion The protective effect of isoflurane on brain is better than that of preconditioning and resuscitation. The protective effect of isoflurane pretreatment and resuscitation are the same.