论文部分内容阅读
目的:利用等位酶分析技术对甘肃野生秦艽的遗传多样性进行分析,为甘肃秦艽的可持续发展和利用提供理论依据。方法:剪取-20℃冰镇的秦艽叶片进行酶的提取、电泳、染色及结果分析。结果:对秦艽的9个野生居群151株个体通过8个酶系统和12个酶位点的分析表明,秦艽具有较低的遗传多样性。每位点平均等位基因数(A)为1.1,多态位点百分率(P)为20.17,秦艽居群间的遗传分化系数(GST)为0.1352,说明其86.48%的遗传变异存在于居群间。结论:秦艽具有较低的遗传多样性并且在其适宜生境中完成自然更新能力较低,应采取以天水、子午岭建立就地保护区为主的综合保护策略。
Objective: To analyze the genetic diversity of wild Gentiana macrophylla from Gansu province by using allozyme analysis technique, and to provide a theoretical basis for the sustainable development and utilization of Gentiana macrophylla in Gansu Province. Methods: Extract Gentiana macrophylla chinensis leaves at -20 ℃ and extract, electrophoresis, staining and analysis of results. Results: The results showed that Gentiana macrophylla had low genetic diversity among 151 individuals of 9 wild populations of Gentiana macrophylla by 8 enzyme systems and 12 enzyme loci. The average number of alleles per locus (A) was 1.1, the percentage of polymorphic loci (P) was 20.17, and the genetic differentiation coefficient (GST) between populations was 0.1352, indicating that 86.48% of the genetic variations existed in populations between. Conclusion: Gentiana macrophylla has a low genetic diversity and low natural regeneration capacity in its suitable habitat. A comprehensive protection strategy based on the establishment of in situ conservation zones in Tianshui and Ziwu Mountains should be taken.