运用GC-MS技术结合AMDIS软件对代谢物种类、含量及其代谢途径进行分析,研究污水与镉复合胁迫对玉米幼苗代谢物的影响.共鉴定出50种代谢物.在镉单一胁迫下,玉米幼苗苹果酸含量降低,柠檬酸含量升高,植株体内三羧酸循环稳定;在污水与镉复合胁迫下,柠檬酸和苹果酸含量均显著增加,复合胁迫阻碍了三羧酸循环的正常进行.镉单一胁迫和污水与镉复合胁迫均导致葡萄糖含量显著下降,与抗逆相关的物质腐胺、脯氨酸、肌醇和γ-氨基丁酸含量均显著升高,表明光合作用受到抑制,但以复合胁迫的变化更为明显.表明污水与镉复合胁迫对玉米幼苗代谢物影响的作用机制主要是通过阻碍三羧酸循环和抑制光合作用进行. The metabolite species, contents and metabolic pathways were analyzed by GC-MS combined with AMDIS software to study the effects of combined wastewater and cadmium stress on the metabolites of corn seedlings. Fifty metabolites were identified. Under single Cd stress, The content of malic acid in the seedling decreased and the content of citric acid increased. The tricarboxylic acid cycle in the plant was stable. Under the combination of sewage and cadmium, the contents of citric acid and malic acid were significantly increased, and the combined stress inhibited the normal progress of tricarboxylic acid cycle. Single stress of cadmium and compound stress of wastewater and cadmium all resulted in a significant decrease of glucose content, contents of putrescine, proline, myo-inositol and γ-aminobutyric acid of substances related to stress resistance were significantly increased, indicating that photosynthesis was inhibited. However, The change of compound stress was more obvious, indicating that the mechanism of the combined effect of wastewater and cadmium stress on metabolites of maize seedlings mainly hindered the tricarboxylic acid cycle and inhibited photosynthesis.