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目的应用寡核苷酸基因芯片技术建立家族不良结局乙型肝炎病毒(HBV)感染者外周血单个核细胞的基因表达谱。方法在一个家族不良结局HBV感染家族中,选取患者5例,患者正常配偶4 例,提取外周血单个核细胞RNA,与涵盖2.2万个ESTs的寡核苷酸表达谱基因芯片U133A 2.0杂交,通过Affymetrix扫描仪和DNT分析软件比较患病组与对照组外周血单个核细胞基因表达谱,获得基因的相对表达比值。结果在2.2万个ESTs中初筛出55个差异表达基因,表达上调14个,表达下调41个,差异基因主要(57%)参与免疫反应、细胞信号转导、细胞周期、代谢、细胞凋亡及炎症基因。结论筛选出的55个基因,是宿主感染HBV的差异表达基因,或宿主对HBV的易感基因,为差异表达基因功能研究建立框架,为宿主HBV易感性研究提供新的靶点。
OBJECTIVE: To establish a gene expression profile of peripheral blood mononuclear cells from patients with poor familial hepatitis B virus (HBV) infection using oligonucleotide microarray technology. Methods In a family familial HBV infection family, 5 patients were selected, 4 patients were normal spouses, RNA of peripheral blood mononuclear cells was extracted and hybridized with U133A 2.0 oligonucleotide microarray gene chip containing 22,000 ESTs, Affymetrix scanner and DNT analysis software to compare the gene expression profiles of peripheral blood mononuclear cells in diseased group and control group to obtain the relative gene expression ratio. Results Fifty-two differentially expressed genes were screened out from 22,000 ESTs, with 14 up-regulated and 41 down-regulated genes. The major differentially expressed genes (57%) were involved in immune response, cell signal transduction, cell cycle, metabolism and apoptosis And inflammatory genes. CONCLUSION: The 55 genes screened out are the differentially expressed genes of host infected with HBV, or the hosts are susceptible genes of HBV, so as to establish a framework for the study of the function of differentially expressed genes and provide a new target for the study of host HBV susceptibility.