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为了得到肝干细胞存在的直接证据, 对小鼠胎肝细胞进行了长期体外培养, 分离到AFP, CD34及ALB等特异性分子标志阳性、并呈集落样生长的原始细胞系. 进而采用体内移植实验, 发现小鼠的脾脏明显增大, 有多个大小不等的结节出现, 而且统计学分析显示, 形成的脾结节数与移植的胎肝细胞数之间呈正相关关系. 组织病理学和免疫组织化学分析表明, 结节中的细胞形态多样, 增殖旺盛, 部分细胞胞质较丰富, 具有肝原始细胞的形态学特征, 而且AFP, CD34等分子标志为阳性. 这些结果证实在小鼠胎肝中存在着具有多分化潜能的肝干细胞.
In order to obtain the direct evidence of the existence of hepatic stem cells, mouse fetal hepatocytes were cultured in vitro for a long time, and the original cell lines with specific molecular markers such as AFP, CD34 and ALB were isolated and presented as colony-like growth.Furthermore, , We found that the spleen of mice increased obviously, there were several nodules of different sizes appeared, and statistical analysis showed that there was a positive correlation between the number of spleen nodules and the number of fetal liver cells transplanted.Histopathology and Immunohistochemical analysis showed that the nodules in the shape of a variety of cells, strong proliferation, some of the cells more abundant cytoplasm with morphological features of hepatoblast cells, and AFP, CD34 and other molecular markers were positive These results confirm that in mouse embryos There are liver stem cells that have pluripotency potential.