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建立小麦条锈菌生理小种的快速分子检测技术体系对小麦条锈菌的监测和防治策略的制定具有重要价值。条锈菌V26是近年来出现的,对我国目前小麦抗病育种中普遍应用的抗条锈病基因Yr26具有毒性的新菌系。该菌系的出现,对我国当前小麦生产、抗病育种都造成了严重威胁。本研究选用189条随机引物对CYR29、CYR31、CYR32、CYR33、T4、Su11-4和V26等7个条锈菌生理小种(菌系)进行了扩增,筛选V26的特异性RAPD片段,并对其进行克隆和测序。根据测序结果,设计并合成SCAR特异性引物,将V26的RAPD标记转化为稳定的SCAR标记。使得对该菌系的快速检测成为可能,同时也将会为条锈菌新小种的监测提供更为准确的科学依据。
The establishment of a rapid molecular detection technology system for wheat stripe rust races is of great value to the monitoring and control strategies of wheat stripe rust. Stripe rust V26 is a new strain that appears in recent years and is toxic to the stripe rust resistance gene Yr26 commonly used in wheat resistance breeding in our country. The appearance of this strain has posed a serious threat to the current wheat production and disease-resistant breeding in our country. In this study, 189 random primers were used to amplify 7 stripe rusty strains (strains) of CYR29, CYR31, CYR32, CYR33, T4, Su11-4 and V26, and to screen the specific RAPD fragment of V26 They were cloned and sequenced. According to the sequencing results, SCAR-specific primers were designed and synthesized, and the RAPD markers of V26 were transformed into stable SCAR markers. Making rapid detection of this strain possible and will also provide a more accurate scientific basis for monitoring the new races of stripe rust.