论文部分内容阅读
目的探讨CpG ODN乙型肝炎疫苗与市售乙型肝炎疫苗对Balb/c小鼠的免疫作用效果。方法将乙肝疫苗、乙肝疫苗+100μgCpG ODN分别肌肉注射到4～6周龄,16～18g Balb/c小鼠体内,于第1次免疫后28d以同样剂量加强免疫1次。分别于第1次免疫后28、42、63 d收集小鼠血清,用ELISA方法检测抗HBs IgG抗体。结果实验组产生的抗HBs IgG与对照组HBsIgG相比P<0.05,具有显著性差异。28、42、63 d抗HBs IgG分别是对照疫苗的114倍、4.73倍、5.41倍以上。结论 CpG ODN能够显著增强小鼠对乙肝疫苗的免疫应答,在较短时间内显著的提高乙肝抗体的产生水平,免疫效果是乙肝疫苗的5倍以上。
Objective To investigate the immune effect of CpG ODN hepatitis B vaccine and commercial hepatitis B vaccine on Balb / c mice. Methods Hepatitis B vaccine and Hepatitis B vaccine + 100μgCpG ODN were intramuscularly injected into 4 ~ 6 weeks old, 16 ~ 18g Balb / c mice respectively, and boosted with the same dose once 28 days after the first immunization. Mouse sera were collected at 28, 42 and 63 days after the first immunization, and anti-HBs IgG antibodies were detected by ELISA. Results The anti-HBs IgG produced by experimental group had significant difference compared with HBsIgG of control group (P <0.05). 28, 42, 63 d anti-HBs IgG were 114 times the control vaccine, 4.73 times, 5.41 times more. Conclusions CpG ODN can significantly enhance the immune response to hepatitis B vaccine in mice, and significantly increase the production of hepatitis B antibody in a relatively short period of time. The immune effect is more than 5 times that of hepatitis B vaccine.