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S-SAP(特异序列扩增多态性,Ssequence specific amplification polymorphism)是一种基于反转录转座元件的广泛应用于生物遗传多样性研究的分子标记。本研究从34对引物中筛选出7对具有谱带清晰、多态性高的引物组合,成功地开发了苹果基因组的S-SAP分子标记。27个元帅系芽变品种中,共扩增出588条谱带,每对引物组合平均扩增出84条谱带,其中多态性谱带48条,多态性谱带占总扩增出谱带数的8.2%,遗传相似系数介于0.73～0.90之间。对15个苹果芽变品种进行S-SAP分析,相似系数在0.42～0.94之间,以相似系数0.80为阈值,可以区分各芽变品系。开发的S-SAP分子标记可以有效地将苹果芽变品种区分,并为苹果生物遗传多样性与系统进化、品种鉴定提供新方法。
S-SAP (Ssequence specific amplification polymorphism) is a molecular marker widely used in biological genetic diversity based on retrotransposon elements. In this study, we selected seven pairs of primers with clear bands and high polymorphism from 34 pairs of primers, and successfully developed the S-SAP molecular marker of apple genome. A total of 588 bands were amplified in 27 Marshal germplasms, with an average of 84 bands per primer pair, of which 48 were polymorphic and the total polymorphism bands were amplified 8.2% of the number of bands, genetic similarity coefficient between 0.73 ~ 0.90. S-SAP analysis of 15 apple bud varieties showed that the similarity coefficients ranged from 0.42 to 0.94, and the similarity coefficient of 0.80 was the threshold value to distinguish the bud varieties. The developed S-SAP molecular marker can effectively differentiate apple bud varieties, and provide a new method for apple biological genetic diversity and phylogeny and species identification.