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本文通过在脂质体内还原金的前体HAuCl_4原位合成了金纳米星.这种设计跟常用的在脂质体内装载金纳米球的方法相比,优点是方便快捷的同时实现了金纳米材料的形成和装载.通过改变实验条件,合成的金纳米星具有可控的尺寸,以及在可见区到近红外区之间的可控吸收光谱.作为一个例子,我们在脂质体内合成了最大吸收在803 nm的金纳米星.这种材料具有温度敏感性,在近红外区可以产生比血液好1.5倍的光声造影信号.当我们把抗癌药物阿霉素装载到这种脂质体内时,近红外区的激光照射可以在10秒内触发药物100%的释放.相对应的,不含纳米星的脂质体在同等条件下只能释放10%的药物,也不具备光声造影的信号增强.最后,我们在癌细胞内测试了该脂质体的疗效,初步验证了该体系的应用前景.
In this paper, gold nanoparticles were synthesized in situ by reduction of HAuCl_4, a precursor of gold in liposomes.Compared with the commonly used methods of loading gold nanospheres in liposomes, the advantage of this design is that it is convenient and fast to realize the gold nanomaterials By changing the experimental conditions, the synthesized gold nanostars have a controllable size, and controllable absorption spectra in the visible to near infrared region As an example, we synthesized in the liposomes maximum absorption Gold nano-stars at 803 nm. This material is temperature-sensitive and produces near 1.5 times better photoacoustic contrast than blood in the near-infrared. When we loaded the anticancer drug doxorubicin into this liposome , Laser irradiation in the near infrared region can trigger 100% release of the drug within 10 seconds, and the corresponding non-nanosized liposomes can only release 10% of the drug under the same conditions and do not have the photoacoustic imaging Signal enhancement.Finally, we tested the efficacy of the liposome in cancer cells, and initially verified the application of the system.