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目的:探讨丹皮酚对动脉粥样硬化(AS)大鼠脂质代谢和核因子-κB(NF-κB)的影响。方法:采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、辛伐他汀组(10 mg·kg-1)、丹皮酚高剂量组和低剂量组(20,10 mg·kg-1),每组8只。灌胃给药4周后,各组取血清,测定血脂水平;取主动脉切片,光镜下作病变分级评分,免疫组化法检测NF-κB的表达。结果:丹皮酚高剂量组能改善AS大鼠的主动脉病变,与模型组比较有统计学意义(P<0.05);丹皮酚高、低剂量组可明显降低AS大鼠血清三酰甘油(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平(P<0.05或P<0.01);丹皮酚可下调主动脉NF-κB表达,高剂量组IOD值(1.96±0.55)和低剂量组IOD值(2.50±0.80)与模型组(3.65±1.14)比较,差异均有统计学意义(P<0.05或0.01)。结论:丹皮酚治疗大鼠AS的作用机制与调节脂质代谢、下调主动脉NF-κB的表达有关。
Objective: To investigate the effect of paeonol on lipid metabolism and nuclear factor-κB (NF-κB) in atherosclerosis rats. Methods: AS model was established by high-fat diet and intraperitoneal injection of vitamin D3. The AS model was divided into normal group, model group, simvastatin group (10 mg · kg-1), paeonol high dose group and low dose Group (20, 10 mg · kg-1), 8 in each group. Four weeks after intragastric administration, the serum was taken from each group and the level of blood lipids was measured. The aorta sections were taken and graded by light microscope. The expression of NF-κB was detected by immunohistochemistry. Results: Paeonol high-dose group can improve the aortic lesion of AS rats compared with the model group (P <0.05); Paeonol high and low dose groups can significantly reduce the serum triglyceride (P <0.05 or P <0.01). Paeonol could down regulate the expression of NF-κB in aorta and IOD in high dose group (1.96 ± 0.55) and the low dose group (2.50 ± 0.80) compared with the model group (3.65 ± 1.14), the difference was statistically significant (P <0.05 or 0.01). Conclusion: The mechanism of paeonol treatment of rat AS is related to the regulation of lipid metabolism and down-regulation of NF-κB in the aorta.