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目的研究Graves病(GD)不同阶段患者外周血叉状头/翅膀状螺旋转录因子(FOXP3)、糖皮质激素诱导肿瘤坏死因子受体(GITR)及IL-2受体α链(CD25)基因的表达变化,探讨其在GD发病机制中的作用。方法收集GD患者90例,按病情分为GD初诊组30例(男13例,女17例);GD缓解组30例(男10例,女20例);GD复发组30例(男11例,女19例)。健康查体者30例(男14例,女16例)作为健康对照组。应用实时荧光定量PCR法检测各组外周血单个核细胞中FOXP3、GITR及CD25mRNA含量,同时利用电化学发光的方法测定各组血清甲状腺激素水平及甲状腺过氧化酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)的水平。结果GD各组患者外周血FOXP3mRNA表达均较健康对照组显著降低(P<0.05),GD缓解组FOXP3mRNA水平较GD初诊组显著升高(P<0.05),复发组FOXP3mRNA水平虽低于缓解组(P<0.05),但明显高于GD初诊组(P<0.05);GD各组女性患者FOXP3mRNA表达水平显著高于男性患者(P<0.05);Graves初诊及复发组GITRmRNA、CD25mRNA表达水平显著高于对照组(P<0.05)。结论FOXP3、GITR及CD35可能参与了Graves的发生、发展及复发过程。
Objective To study the expression of FOXP3, GITR and CD25 genes in patients with Graves’ disease (GD) at different stages. Expression changes, to explore its role in the pathogenesis of GD. Methods Ninety patients with GD were collected and divided into GD newly diagnosed group (30 males and 13 females) according to their disease; 30 patients (10 males and 20 females) in GD remission group; 30 patients (GD 11) , 19 females). Health examination of 30 cases (14 males and 16 females) as a healthy control group. The levels of FOXP3, GITR and CD25mRNA in peripheral blood mononuclear cells of each group were detected by real-time fluorescence quantitative PCR. The level of serum thyroid hormone and the levels of thyroid hormone (TPOAb), thyroglobulin antibody (TGAb) levels. Results The expression of FOXP3mRNA in peripheral blood of GD patients was significantly lower than that of healthy controls (P <0.05), while the level of FOXP3 mRNA in GD remission group was significantly higher than that of newly diagnosed GD patients (P <0.05) (P <0.05), but was significantly higher than that of the newly diagnosed group (P0.05). The FOXP3 mRNA expression levels in female patients with GD were significantly higher than those in male patients (P0.05). The expression of GITR mRNA and CD25mRNA in the newly diagnosed and relapsed Graves group was significantly higher than Control group (P <0.05). Conclusion FOXP3, GITR and CD35 may participate in the development, progression and relapse of Graves.