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目的了解沙门氏中腹泻病人便样本中的携带及感染状况,探讨食源性沙门氏菌与临床病例之间的关系,为由其引起的食物中毒病原及食品中沙门氏菌污染状况调查提供参考。方法采集本辖区两个哨点医院门诊腹泻患者病例标本,用增菌液和培养基进行增菌和分离培养,用生化鉴定试剂盒及血清学实验进行沙门氏菌鉴定。结果在240例腹泻病人标本中,检出5株沙门氏菌,检出率2.08%。结论腹泻病人标本中能够检出沙门氏菌,为了提高感染人群标本中沙门氏菌检出率,需要进一步优化分离培养条件。
Objective To understand the status of carriage and infection of Salmonella in diarrhea patients and to explore the relationship between foodborne Salmonella and clinical cases and to provide references for the investigation of the food poisoning pathogens and the contamination of Salmonella in foodstuffs. Methods The samples of diarrhea patients from outpatients of two sentinel hospitals in the area were collected, and the bacteria and culture medium were added for enrichment and isolation. Salmonella was identified by biochemical identification kit and serological test. Results In 240 samples of diarrhea patients, 5 strains of Salmonella were detected, the detection rate was 2.08%. Conclusion Salmonella can be detected in diarrhea patients. In order to improve the detection rate of Salmonella in infected samples, the conditions of isolation and culture should be further optimized.