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目的:研究孔雀柏(Chamaecyparis obtusa cv.Tetragon)的化学及活性成分。方法:综合运用工业甲醇提取、碱溶酸沉,通过硅胶柱、Sephadex LH-20、C18反相硅胶以及制备液相等色谱方法分离纯化,并采用MS和NMR等波谱学方法鉴定化合物结构,测试各化合物的细胞毒活性。结果:分离并鉴定了8个化合物,分别为catechin(1)、taxifolin-3-O-β-D-xylopyranoside(2)、icariside E4(3)、(-)arctigenin(4)、savinin(5)、horsfieldin(6)、dihydrosesamin(7)和skimmin(8)。结论:此8个化合物均为在该植物中首次分离得到。体外细胞毒活性筛选表明,孔雀柏的甲醇提取物及其总生物碱部位在体外对人肿瘤细胞株A549、BGC-823、K562均具有一定的细胞毒活性。此8个化合物均无明显细胞毒活性,进一步活性测试和化学研究尚在进行中。
Objective: To study the chemical and active ingredients of Chamaecyparis obtusa cv.Tetragon. Methods: The compounds were isolated and purified by silica gel column, Sephadex LH-20, C18 reversed-phase silica gel and preparative liquid chromatography. The structures of the compounds were identified by MS, NMR and other spectroscopic methods. Cytotoxic activity of each compound. RESULTS: Eight compounds were isolated and identified as catechin (1), taxifolin-3-O-β-D-xylopyranoside (2), icariside E4 (3), (-) arctigenin (4), savinin (5) , Horsfieldin (6), dihydrosesamin (7) and skimmin (8). Conclusion: All the eight compounds were isolated from this plant for the first time. In vitro cytotoxic activity screening showed that the methanolic extract of Peacockii and its total alkaloids site in vitro on human tumor cell lines A549, BGC-823, K562 have a certain degree of cytotoxic activity. All 8 compounds showed no obvious cytotoxic activity, and further activity testing and chemical research are still underway.