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目的研究罗汉松实(花托)的急性毒性和亚慢性毒性,并对其安全性进行评价。方法急性毒性试验采用最大给药量法,以160.0 g/(kg·d)的剂量分2次小鼠灌胃200%罗汉松花托汁,观察14 d内的毒性反应。亚慢性毒性试验分3组,实验组分别以20 g/kg和10 g/kg的剂量,SD大鼠灌胃200%罗汉松花托汁,对照组给予蒸馏水,连续灌胃90 d。实验结束时采血测血液学和血生化指标并进行组织病理学检查,计算脏器系数。结果各组动物未见明显毒性反应,也未见死亡。实验组脏器系数、血液学和血生化指标与对照组相比,差异均无统计学意义(P>0.05)。组织病理学检查也未见与受试物相关的病理学改变。结论在本实验条件下,未见罗汉松花托汁对实验动物有明显的毒性反应,罗汉松花托安全性较高,可作为食品原料开发利用。
Objective To study the acute toxicity and subchronic toxicity of Podocarpaceae (Torula) and to evaluate its safety. Methods Acute toxicity test was conducted in the maximum dose of 200% (200.0 g / (kg · d)). The mice were orally administered 200% Luohuosong pod juice at a dose of 160.0 g / (kg · d) for 14 days. The sub-chronic toxicity test was divided into three groups. The rats in the experimental group were treated with 20 g / kg and 10 g / kg respectively, while the SD rats were treated with 200% Luohuosong pod juice. The control group was given distilled water continuously for 90 days. At the end of the experiment, hematological and blood biochemical indexes were measured by blood sampling and histopathological examination was performed to calculate the organ coefficient. Results The animals in each group showed no obvious toxic reaction and no death was found. There was no significant difference in the organ coefficient, hematology and blood biochemistry between the experimental group and the control group (P> 0.05). No histopathological changes associated with the test substance were found in the histopathological examination. Conclusions Under the experimental conditions, there is no obvious toxic reaction of Luohuosong pod juice to the experimental animals. The Luohuasong torus is safe and can be used as a food raw material.