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通过细胞增殖实验(MTT)研究不同浓度芦荟大黄素(Aloe emodin,AE)对人宫颈癌细胞Si Ha生长的抑制作用,并将Si Ha细胞注入48 hpf的斑马鱼胚胎卵黄囊,建立斑马鱼移植瘤模型,然后用不同浓度AE进行处理,考察肿瘤细胞在斑马鱼体内的增殖和迁移情况。结果显示,AE对Si Ha细胞的生长抑制率随浓度和作用时间的增加而呈明显上升趋势,表现出一定的浓度-时间-效应依赖关系;体内实验表明AE对斑马鱼移植瘤的增殖和转移具有抑制作用。本研究表明,AE具有开发成抗宫颈癌药物的潜能,同时斑马鱼移植瘤模型也可以成为宫颈癌研究和药物评价的重要模型。
Cell proliferation assay (MTT) was used to study the inhibitory effect of Aloe emodin (AE) on the growth of human cervical carcinoma Si Ha cells. Si ha cells were injected into the zebrafish embryo yolk sac of 48 hpf to establish zebrafish transplantation Tumor model, and then treated with different concentrations of AE to examine the proliferation and migration of tumor cells in zebrafish. The results showed that the inhibitory rate of AE on the growth of Si Ha cells increased with the increase of concentration and duration of action, showing a certain concentration-time-effect dependence. In vivo experiments showed that AE inhibited the proliferation and metastasis of zebrafish xenografts With inhibition. This study shows that AE has the potential to develop anti-cervical cancer drugs and that zebrafish xenograft models can also be an important model for cervical cancer research and drug evaluation.