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分析鼻咽癌常规 H.E染色细胞涂片分别作 Feulgen染色检测 DNA含量和原位杂交检测 EB病毒编码 RNAs(EBERs)的效果 ;将常规 HE染色的 9例正常鼻咽细胞涂片和 38例鼻咽癌细胞涂片用 1%酸性酒精褪色 ,然后作 Feulgen染色 ,应用图象分析仪测定涂片细胞 DNA含量 ,并将 38例鼻咽癌细胞病例的阳性涂片进行 EBERs原位杂交检测 ;结果显示正常鼻咽细胞均为 DNA二倍体核型 ,38例癌阳性涂片中呈 DNA二倍体者 6例 ,DNA非二倍体者 32例 (84.2 % ) ,癌细胞核 EBERs阳性者 35例 (92 .1% ) ;结果表明 HE染色的鼻咽癌细胞学涂片经褪色后作 Feulgen染色和原位杂交检测 ,能较满意进行 DNA和 EBERs分析 ,表明常规 H.E染色和褪色处理均不影响 Feulgen染色和 EB病毒原位杂交的质量效果。本检测方法可靠、可行 ,适用于常规染色细胞学样本的回顾性研究和随访研究 ,并可用于鼻咽癌可疑病人的诊断和鉴别诊断
Analysis of routine HE staining of nasopharyngeal carcinoma cell smears were detected by Feulgen staining DNA content and in situ hybridization detection of Epstein-Barr virus RNAi (EBERs) effect; routine HE stained in 9 cases of normal nasopharyngeal smear and 38 cases of nasopharyngeal The smear of cancer cells was faded with 1% acidic alcohol and then stained with Feulgen. The DNA content of the smear cells was determined by image analyzer. The positive smears of 38 nasopharyngeal carcinoma cells were detected by EBERs in situ hybridization. The results showed Normal nasopharyngeal cells were DNA diploid karyotype, 38 cases of cancer-positive smears were DNA diploid in 6 cases, 32 cases of DNA non-diploid in 32 cases (84.2%), 35 cases of nuclear EBERs were positive 92.1%). The results showed that smear of HE-stained nasopharyngeal carcinoma smear after staining by Feulgen staining and in situ hybridization, can be more satisfactory for DNA and EBERs analysis showed that conventional HE staining and fade treatment did not affect Feulgen Quality effect of staining and EB virus in situ hybridization. The detection method is reliable and feasible, suitable for routine staining cytological samples of retrospective study and follow-up study, and can be used for the diagnosis and differential diagnosis of suspicious patients with nasopharyngeal carcinoma