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目的了解上海浦东新区2012—2015年间诺如病毒GII型亚型感染情况,为诺如病毒GII型的研究和诺如病毒感染引起的聚集性腹泻检测提供依据。方法对2012年7月—2015年6月间区内14家哨点医院检测结果为诺如病毒GII型阳性的粪便样本,应用RT-PCR扩增诺如病毒VP1蛋白ORF2部分基因并测序,通过MEGA 7.0软件分析诺如病毒GII型的变异情况。结果在1231份诺如病毒GII型阳性样本中,有863份成功扩增并测序。通过分型,发现该地区在监测时段内,有GII.1、2、3、4、5、6、7、8、12、13、14、17,21共13个诺如病毒GII型亚型存在。GII.4和GII.17亚型是诺如病毒GII型的主要流行亚型并且,自2014年8月起,诺如病毒GII.17亚型逐渐取代GII.4亚型,成为本地区诺如病毒GII型的主要流行亚型。结论诺如病毒GII型是本地区聚集性腹泻暴发和流行的主要病原,对诺如病毒GII型亚型的研究,不仅为诺如病毒进化研究提供了样本基础,并且为诺如病毒的精确防控提供了理论依据。
Objective To understand the status of norovirus GII subtype infection in Shanghai Pudong New Area from 2012 to 2015 and provide basis for the study of Norovirus type GII and the detection of aggregated diarrhea caused by norovirus infection. Methods From July 2012 to June 2015, fourteen sentinel-affiliated hospitals were tested for norovirus GII-positive stool samples. RT-PCR was used to amplify and sequenced the ORF2 gene of norovirus VP1 protein. MEGA 7.0 software analyzes the variation of norovirus GII. Results In 1231 Norovirus GII-positive samples, 863 were successfully amplified and sequenced. By typing, we found that in the monitoring period, there are 13 norovirus GII subtypes of GII.1,2,3,4,5,6,7,8,12,13,14,17,21 exist. The GII.4 and GII.17 subtypes are major epidemic subtypes of norovirus GII and since August 2014, the GII.17 subtype of norovirus has gradually replaced the GII.4 subtype, The major epidemic subtype of virus GII. Conclusions Norovirus GII is the major causative agent of the outbreak and epidemic of cluster diarrhea in the region. The study of Norovirus GII subtypes not only provided a sample basis for Norovirus evolutionary studies, Control provides a theoretical basis.