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目的研究ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中Ｔ细胞的异常、异常Ｔ细胞的起源及其与淋巴腺病理的关系。方法取出生后不同时期ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠及正常Ｃ５７ＢＬ／６小鼠的造血淋巴组织，制备单细胞悬液并用流式细胞技术检测其细胞表面标记，然后以ｔ检验进行统计学处理。结果发现ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中存在着一个异常的、与淋巴腺病理密切相关的Ｔ细胞亚群，其表型为Ｔｈｙ１．１－ＴＣＲαβ＋Ｌｙ５＋，而且这一细胞亚群随着年龄增长而迅速增加，至１９周龄，其可以占淋巴结细胞总数的８０％。同时，淋巴结及脾脏中传统Ｔ细胞（Ｔｈｙ１．１＋ＴＣＲαβ＋Ｌｙ５－）随着Ｔｈｙ１．１－ＴＣＲαβ＋Ｌｙ５＋细胞的增加而逐渐减少。结果还表明，这一细胞亚群既不在胸腺也不在骨髓中分化成熟，但这一细胞亚群起源于某些异常骨髓造血细胞。结论ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中存在着一个起源于某些异常骨髓造血细胞的表型为Ｔｈｙ１．１－ＴＣＲαβ＋Ｌｙ５＋的随着年龄增长而迅速增加的Ｔ细胞亚群
Objective To investigate the abnormalities of T cells and the origin of abnormal T cells in MRL / lprThy1.1 mice and their relationship with the pathology of lymph nodes. Methods The hematopoietic lymphoid tissue of MRL / lprThy1.1 mice and normal C57BL / 6 mice were taken out from different periods after birth to prepare single cell suspension. The cell surface markers were detected by flow cytometry and then analyzed by t test. The results showed that there was an abnormal T lymphocyte subsets in MRL / lprThy1.1 mice which was closely related to lymphoid pathology and its phenotype was Thy1.1-TCRαβ + Ly5 +, and this subpopulation of cells rapidly with age Increase to 19 weeks of age, which can account for 80% of the total lymph node cells. Meanwhile, the number of T lymphocytes (Thy1.1 + TCRαβ + Ly5-) in lymph nodes and spleen decreased with the increase of Thy1.1-TCRαβ + Ly5 + cells. The results also indicate that this subset of cells neither differentiate in the thymus nor in the bone marrow, but this subpopulation of cells originates from certain abnormal bone marrow hematopoietic cells. Conclusions There is a T cell subpopulation in MRL / lprThy1.1 mice with a rapidly increasing phenotype of Thy1.1-TCRαβ + Ly5 +, which is derived from certain abnormal bone marrow hematopoietic cells.