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目的:探索影响未成熟卵母细胞冻融技术的关键因素。方法:A组:来源于体外受精-胚胎移植(IVF-ET)周期中自愿捐献的多余的成熟卵母细胞(MⅡ期),共56个;B组:来源于手术切除的卵巢组织中的未成熟卵母细胞(GV或MⅠ),共67个。不同成熟时期卵母细胞经慢冻快融后培养和体外受精,观察其体外成熟、受精及胚胎发育情况。结果:A组与B组相比,两组冻融后的存活率有显著性差异(60.71%vs 77.61%,P<0.05);A组受精率高于B组,但两组比较无显著性差异(61.76%vs 50.00%,P>0.05);A组与B组相比,两组冻融后的卵裂率和优质胚胎率有显著性差异(76.19%vs 37.50%,47.62%vs 12.50%,P<0.05)。A组获2枚囊胚,而B组没有囊胚培养成功。结论:体外成熟培养技术可能是影响冻融后未成熟卵母细胞发育的关键因素。
Objective: To explore the key factors affecting the freezing and thawing of immature oocytes. Methods: Group A: Excess mature oocytes derived from voluntary donation during IVF-ET cycles (MII stage), 56 in total; Group B: ovarian tissue from resected ovaries Mature oocytes (GV or MI), a total of 67. Oocytes at different stages of maturation were cultured and in vitro fertilized by slow freezing, and their in vitro maturation, fertilization and embryo development were observed. Results: The survival rate of group A and group B after freezing and thawing was significantly different (60.71% vs 77.61%, P <0.05). The fertilization rate of group A was higher than that of group B, but there was no significant difference between the two groups (61.76% vs 50.00%, P> 0.05). Compared with group B, the cleavage rate and quality embryo rate of the two groups were significantly different (76.19% vs 37.50%, 47.62% vs 12.50% , P <0.05). Two blastocysts were obtained in group A, but no blastocysts were successfully cultured in group B. Conclusion: In vitro maturation culture may be the key factor that affects the development of immature oocytes after freezing and thawing.