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目的 进一步明确北京林区是否存在莱姆病的自然疫源地及其分布。方法 基于莱姆病螺旋体外膜蛋白A基因建立半巢式聚合酶链式反应 (polymerasechainreaction ,PCR)方法 ,对从北京 6个林区采集的蜱和鼠进行检测和基因分型 ,选择阳性标本进行克隆和序列测定 ,与已知序列进行同源性比较。间接免疫荧光法检测抗莱姆病螺旋体IgG抗体 ,从长角血蜱中分离莱姆病螺旋体。结果 从门头沟区东灵山采集的标本中检测到莱姆病螺旋体DNA片段 ,3只游离全沟硬蜱 1只检测阳性 ,5 7只寄生全沟硬蜱若蜱中 1只检测阳性 ;119只野鼠中 9只检测阳性 ,其中 8只B .garinii阳性 ,1只B .afzelii阳性。 5 0份野鼠血清有 5份莱姆病螺旋体IgG抗体阳性 ,采集的 16 0只长角血蜱(2 0只 /组 )未分离到莱姆病螺旋体菌株。结论 北京门头沟区东灵山可能存在莱姆病的自然疫源地 ,包括两个基因型 ,全沟硬蜱可能是莱姆病的传播媒介 ,野鼠可能是贮存宿主。
Objective To further clarify whether there is a natural foci of Lyme disease in Beijing’s forest area and its distribution. Methods Based on the polymerase chain reaction (PCR) method of Helicobacter pylori spirometic protein A gene, ticks and mice collected from 6 forest areas in Beijing were detected and genotyped. The positive samples were selected Clone and sequence analysis, homology comparison with known sequences. Indirect immunofluorescence assay for detection of anti - Lyme disease spirochete IgG antibody, Borrelia burgdorferi isolated from Helicoverpa armigera. Results A DNA fragment of Borrelia burgdorferi was detected in specimens collected from Dongling Mountain, Mentougou District. One of three free-iaped whole hard ticks was positive, and one of the 57 ticks Of the 9 tested positives, 8 were B. garinii positive and 1 B. afzelii positive. Fifty copies of Lyme disease Helicobacter pylori positive IgG antibodies were detected in 50 wild animals serum samples. No spores of Lyme disease were isolated from 160 parasites collected. Conclusion There may be natural foci of Lyme disease in Dongling Mountain, Mentougou District of Beijing, including two genotypes. Ixodes fortunei may be the carrier of Lyme disease. Vole may be the storage host.