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目的建立同时测定芫花药材中棕榈酸和亚油酸含量的气相色谱分析方法。方法采用毛细管气相色谱法,甲酯化后测定芫花中棕榈酸与亚油酸的含量。采用DB-17石英毛细管柱(30 m×0.25 mm,0.25μm);氢火焰离子化检测器(FID);程序升温:起始温度180℃,以5℃.min-1升至230℃;进样口温度:250℃;检测器温度:270℃;载气为氮气,流速:1.2 mL.min-1;分流比为20∶1。结果棕榈酸甲酯和亚油酸甲酯的质量浓度分别在0.079~1.578 g.L-1(r=0.999 6)和0.029~0.590 g.L-1(r=0.999 7)内与峰面积呈良好的线性关系;平均回收率(n=9)分别为98.1%(RSD=2.7%)和99.7%(RSD=2.8%)。结论本方法简便、快速、准确,为芫花的质量控制方法提供依据。
Objective To establish a gas chromatographic method for simultaneous determination of palmitic acid and linoleic acid in Daphne genkwa. Methods The contents of palmitic acid and linoleic acid in Daphne genkwa were determined by capillary gas chromatography after methyl esterification. Using a DB-17 quartz capillary column (30 m × 0.25 mm, 0.25 μm); hydrogen flame ionization detector (FID); programmed temperature rise: initial temperature of 180 ° C, Sample temperature: 250 ° C; detector temperature: 270 ° C; carrier gas was nitrogen, flow rate: 1.2 mL.min-1; split ratio 20: 1. Results The mass concentration of methyl palmitate and methyl linoleate showed a good linear relationship with the peak area in the range of 0.079-1.578 gL-1 (r = 0.999 6) and 0.029-0.590 gL-1 (r = 0.999 7) The average recovery (n = 9) was 98.1% (RSD = 2.7%) and 99.7% (RSD = 2.8%), respectively. Conclusion The method is simple, rapid and accurate, providing the basis for quality control of Daphne genkwa.