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以承德红蘑和白蘑子实体为试材,采用真菌通用引物ITS1和ITS4,对其基因组rDNA基因的ITS区段进行PCR扩增、克隆测序,而后与GenBank数据库信息比对,并利用MEGA 5.0软件构建系统发育树,从分子水平上鉴定其种属地位及系统进化关系。结果表明:承德红蘑与血红铆钉菇(Chroogomphus rutilus,Accession No.:KR082876)、白蘑与Hygrophorus queletii(Accession No.:JF908069)的相似性均达到99%。承德红蘑与血红铆钉菇C.rutilus(Accession No.:KR082876),承德白蘑与H.queletii(Accession No.:JF908069)的遗传关系最近,遗传距离为0.002。因此,承德红蘑和白蘑经分子鉴定为C.rutilus和H.queletii,其分类地位的解析,对今后野生红蘑和白蘑种质资源鉴定、生物学特性研究及开发利用奠定了基础。
Using Chengde red mushroom and white mushroom as test material, ITS1 and ITS4 of common fungi were used to amplify the ITS region of the genomic rDNA gene, then cloned and sequenced, and then compared with the GenBank database, and the MEGA 5.0 The software constructs phylogenetic tree, which identifies its species status and phylogenetic relationship at the molecular level. The results showed that the similarities between Chengde red mushroom and Chroogomphus rutilus (Accession No.:KR082876) and white mushroom and Hygrophorus queletii (Accession No.:JF908069) all reached 99%. C. rutilus (Accession No.:KR082876), Chengde white mushroom and H.queletii (Accession No.:JF908069) had the closest genetic relationship with a genetic distance of 0.002. Therefore, Chengde red mushroom and white mushroom molecular identification as C.rutilus and H.queletii, its classification status analysis, the future of wild red mushroom and white mushroom germplasm identification, biological characteristics of the research and development and utilization laid the foundation.