为探讨寄生虫与癌变的关系,作者用细菌检测方法评价了日本血吸虫和华支睾吸虫的抽提物的可能诱变活性和促肿瘤活性。抽提物制备:从小鼠门静脉和小肠获得的日本血吸虫(Kofu株)成虫和虫卵分别加5.8mmol/L PBS液,用Teflon杵棒玻璃匀浆器匀化,超声处理,再经6×10~4×g,4℃条件下离心30分针,所得上清液冰冻备用。华支睾吸虫成虫处理方法同上。用Lowry法测定蛋白浓度,并用经共价偶联铜酞菁的“蓝棉”处理,除去可能引起突变的多环类化合物。突变试验:采用鼠伤寒沙门氏菌TA98, In order to explore the relationship between parasites and carcinogenesis, the authors evaluated the possible mutagenic activity and tumor-promoting activity of extracts of Schistosoma japonicum and Clonorchis sinensis by bacterial detection. Extract preparation: Adults and worms of Schistosoma japonicum (Kofu strain) obtained from mouse portal vein and small intestine were respectively treated with 5.8 mmol / L PBS solution, homogenized with a Teflon pestle glass homogenizer, sonicated and then subjected to 6 × 10 ~ 4 × g, centrifuged at 4 ℃ 30 minutes, the resulting supernatant frozen. Clonorchis sinensis Adult treatment method Ibid. The protein concentration was determined by the Lowry method and treated with “blue cotton” covalently coupled with copper phthalocyanine to remove the polycyclic compounds that could cause mutations. Mutation test: Salmonella typhimurium TA98,