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DELLA蛋白是GA信号途径中重要的负向调控因子,能够响应各种环境信号,在植物抵抗生物和非生物胁迫中起着重要的作用。功能获得性DELLA突变体矮变1号在矮化育种上已得到广泛应用,但是其在耐盐方面的研究鲜有报道。本文采用含200 mmol L~(–1) NaCl的Hoagland水培溶液处理中国春、京411、矮秆早和矮变1号幼苗7 d,测定盐胁迫下的总叶绿素含量、相对含水量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并借助免疫印迹检测了4个小麦品种中DELLA蛋白的积累。盐胁迫处理后,矮变1号的叶片没有出现明显的萎蔫和失绿,而其他3个品种均表现出不同程度的萎蔫。盐胁迫使4个小麦品种的总叶绿素含量和相对含水量均有所下降,但矮变1号的下降幅度最小;与此同时,矮变1号体内SOD活性的相对上升幅度最大,并且体内的MDA含量相对上升幅度最小。4个品种中矮变1号的DELLA蛋白积累量最高,矮变1号具有较高的耐盐性与其体内的高DELLA蛋白含量密切相关。
DELLA protein is an important negative regulator in GA signal pathway, which can respond to various environmental signals and play an important role in plant resistance to biological and abiotic stresses. The gain-of-function DELLA mutant Dwarf No. 1 has been widely used in dwarf breeding, but few studies have reported its salt tolerance. In this paper, Hoagland hydroponics solution containing 200 mmol L ~ (-1) NaCl was used to treat Chinese spring, Beijing 411, dwarf early and dwarfing seedlings for 7 days, and the total chlorophyll content, relative water content, SOD activity and malondialdehyde (MDA) content were determined. The accumulation of DELLA protein in four wheat cultivars was detected by Western blot. After salt stress treatment, there was no obvious wilting and chlorosis in the leaves of Dwarfing No.1, while the other three varieties showed different degrees of wilting. Under salt stress, the total chlorophyll content and relative water content of four wheat cultivars decreased, but the decrease of Dwarfing No.1 was the lowest. At the same time, the relative increase of SOD activity in Dwarfing No.1 was the highest, MDA content of the relative increase in the smallest. Among 4 varieties, Dwarf No.1 had the highest accumulation of DELLA protein, while Dwarf No.1 had higher salt tolerance and higher DELLA protein content.