革兰阴性菌肺炎是最常见的院内肺炎之一,病死率可高达50%以上。及时诊断和有效使用抗生素治疗能够降低病死率,因此具有重要临床意义[1]。我们从1997年1月起开展了采用鲎细胞溶解物试验(LAL)检测支气管肺泡灌洗液(BALF)内毒素,以协助快速诊断革兰阴性菌肺炎的前瞻性试验,取得了 Gram-negative bacteria pneumonia is one of the most common hospital pneumonia, with a mortality rate of up to 50%. Timely diagnosis and effective use of antibiotic treatment can reduce mortality, so has important clinical significance [1]. We conducted a prospective trial to detect endotoxin in bronchoalveolar lavage fluid (BALF) using the Cytolytic Assay (LAL) to assist in the rapid diagnosis of Gram-negative pneumonia from January 1997 and obtained