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以不同基因型甘蔗品种新台糖22号、新台糖16号、桂糖21号及拔地拉为供试材料,以近生长点0～3 cm的嫩叶鞘组织为外植体、MS固体培养基为基本培养基,研究诱导初期不同分化刺激培养时间和不同激素组合(2,4-D 1.0～7.0 mg/L、ABA 0～2.0 mg/L)对愈伤组织胚性细胞团诱导或分化的影响。结果表明,在MS+6-BA 1.5 mg/L+NAA 0.5mg/L培养基中对嫩叶鞘愈伤组织进行分化刺激培养24～72 h,可显著提高胚性细胞团的诱导率,以培养48 h的诱导率最高,平均诱导率为44.4%,比对照增加15.7个百分点;在不同激素组合培养基中,以在培养基MS+2,4-D 3.0 mg/L+ABA0.5 mg/L中培养的不同甘蔗品种胚性细胞团诱导率较高、生长较好,其次是MS+2,4-D 3.0 mg/L;在分化培养基MS+6-BA 1.5 mg/L+NAA 0.1 mg/L中对来自不同诱导培养基的胚性细胞团进行分化培养,结果发现在MS+2,4-D 3.0 mg/L+ABA 0.5 mg/L和MS+2,4-D 3.0 mg/L中诱导的胚性细胞团的绿苗分化率较高,不同甘蔗品种平均绿苗分化率分别为65.0%、64.9%,但绿苗生根率较低。
Using different genotypes of sugarcane variety, Xintaandu 22, Xintai 16, Guitang 21 and Putila as test materials, young plantlets with 0 ~ 3 cm growing point were used as explants. MS solid medium was The effects of 2,4-D 1.0 ~ 7.0 mg / L and ABA 0 ~ 2.0 mg / L on the induction or differentiation of embryogenic callus were studied in the initial culture medium with different differentiation stimuli and different hormone combinations . The results showed that induction of embryogenic callus induction could be significantly enhanced by differentiation and differentiation of young leaf sheath callus cultured for 24-72 h in MS + 6-BA 1.5 mg / L + NAA 0.5 mg / L medium The induction rate of 48 h was the highest, with the average induction rate of 44.4%, which was 15.7% higher than that of the control. The medium of different hormones combined with MS + 2,4-D 3.0 mg / L + ABA 0.5 mg / L and MS + 2,4-D 3.0 mg / L, respectively. In the medium of MS + 6-BA 1.5 mg / L + NAA 0.1 The results showed that MS / 2,4-D 3.0 mg / L + ABA 0.5 mg / L and MS + 2,4-D 3.0 mg / L, the green plantlet differentiation rate of embryogenic cell mass was higher, and the average green plantlet differentiation rate of different sugarcane varieties was 65.0% and 64.9% respectively, but the green plantlet rooting rate was lower.