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亲合性染料配位体汽巴蓝F3GA在碱性条件下通过三嗪环氯化物和羟乙基甲基丙烯酸酯(HEMA)羟基基团之间的亲核反应与聚乙二醇二甲基丙烯酸酯—羟乙基甲基丙烯酸酯(EGDMA-HEMA)微球共价键偶合,将2价锌离子引入每克聚合物附着有16.5μmol汽巴蓝F3GA的微球中,每克微球载有198.6μmol的2价锌离子,我们在填充柱中使用附着有汽巴蓝F3GA-Zn的亲合性吸附剂从水溶液和人体血浆中吸附白蛋白,微球对牛的血清白蛋白(BSA)的吸附量随着再循环速度的加快而降低,我们观察到开始时吸附速率很高,然后在约60min时逐渐达到平衡,移动相中BSA的浓度也会对吸附产生影响,微球对BSA的吸附量首先随BSA浓度的增高而增大,然后在每克微球吸附约120mg的BSA时达到一平衡状态,在pH值为5.0时我们观察到了微球最大的吸附量,该pH值是BSA的等电位pH值,我们观察到微球对人体血浆中的血清白蛋白具有较高的吸附量(每克微球吸附215mg人体血清白蛋白),使用1.0M的硫氰化物(pH为8.0)在30min内即可达到很高的吸附(已被吸附的白蛋白超过90%)速率。
The affinity dye ligand Cibambar F3GA undergoes a nucleophilic reaction between a triazine ring chloride and a hydroxyethylmethacrylate (HEMA) hydroxyl group under basic conditions with polyethylene glycol dimethacrylate Covalent coupling of ester-hydroxyethylmethacrylate (EGDMA-HEMA) microspheres, divalent zinc ions were introduced into microspheres with 16.5 μmol of Ciba blue F3GA per gram of polymer attached per gram of microspheres 198.6 μmol of divalent zinc ions, we adsorbed albumin from aqueous solution and human plasma using an affinity adsorbent with Ciba blue F3GA-Zn attached to the packed column. The microspheres adsorbed bovine serum albumin (BSA) The amount of adsorption decreased with the recycle rate. We observed that the adsorption rate was high at the beginning, and then gradually reached equilibrium at about 60 min. The concentration of BSA in the mobile phase also had an effect on the adsorption. The adsorption of BSA by the microspheres The amount of BSA increased firstly with the increase of BSA concentration and then reached an equilibrium state with about 120 mg BSA adsorbed per gram of microspheres. At pH 5.0, we observed the maximum amount of adsorbed microspheres, which is BSA Equipotential pH, we observed microspheres on human serum albumin has more (Adsorption of 215 mg of human serum albumin per gram of microspheres), a very high rate of adsorption (over 90% of adsorbed albumin) can be achieved within 30 min using 1.0 M thiocyanate (pH 8.0) .