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酶在毒剂、毒物分析中的应用日益广泛。因其具有方法灵敏、专一,操作简便、快速等优点,因此深受毒剂检验工作者的重视。一、神经性毒剂和有机磷农药的检定胆碱酯酶能催化橙红色的2,6-二氯乙酰靛酚染料水解为蓝色的2,6-二氯靛酚和醋酸。当水中有神经性毒剂或有机磷农药时,胆碱酯酶的活力被抑制,催化2,6-二氯乙酰靛酚水解的速度便减慢,因此,以空白作对照,观察其颜色变化,即可加以区别。检定灵敏度:V类毒剂0.03μg/ml,G类毒剂0.07～0.2μg/ml,有机磷农药0.6～10μg/ml。胆碱酯酶能催化硫代乙酰胆碱水解为硫胆碱和醋酸。当有蓝色的2,6-二氯靛酚存在时,靛酚染料被硫胆碱还原,由蓝色逐渐褪成无色,若水中有神经性毒剂或有机磷农药时,胆碱酯酶的活力被抑制,催化硫代乙酰胆碱水解的能力降低,2,6-二氯靛酚染料褪色速度减慢,以空白作对照,根据在一定时间内靛酚染料褪色程度不同,即可以检定有无神经
Enzyme in the toxicology, the analysis of the increasingly widespread use. Because of its sensitive, specific, easy to operate, fast and so on, so by the toxicity test workers attention. First, the detection of neurotoxic agents and organophosphorus pesticides Cholinesterase catalyzes the hydrolysis of the orange-red 2,6-dichloroacetyl-indophenol dye to blue 2,6-dichloro-indophenol and acetic acid. When there is neurotoxic agent or organophosphorus pesticide in water, the activity of cholinesterase is inhibited and the rate of hydrolysis of 2,6-dichloroacetylindophenol is slowed down. Therefore, the blank is used as control to observe the color change, Can be distinguished. Test sensitivity: V class toxic 0.03μg / ml, G class toxicant 0.07 ~ 0.2μg / ml, organophosphorus pesticide 0.6 ~ 10μg / ml. Cholinesterase catalyzes the hydrolysis of thioacetylcholine to thiocholine and acetic acid. When there is blue 2,6-dichloro-indophenol, the indophenol dye is reduced by thioline and gradually faded from blue to colorless. If there is neurotoxic or organophosphorus pesticide in water, cholinesterase Of the activity is inhibited, the ability to catalyze the hydrolysis of thioacetyl reduced, 2,6-dichloro-indophenol dye fading slowed to the blank as a control, according to a certain period of indophenol dye fade degree is different, you can check the presence or absence nerve