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目的 了解“净常乐牌水苏糖冲剂”对实验小鼠的免疫调节作用。方法 采用《保健食品功能学评价程序和检验方法》 (卫生部 1997年 )中免疫调节作用检验方法进行。结果“净常乐牌水苏糖冲剂”在 0 2 5g/(kg bw)～ 1 0 0g/(kg bw)剂量范围内 ,经 3 0d灌胃小鼠 ,细胞免疫功能测定结果为阴性 :绵羊红细胞 (SRBC)诱导小鼠迟发型变态反应 (DTH)实验结果为阳性 (P <0 0 1) ,ConA诱导的小鼠脾淋巴细胞转化实验 (MTT)结果为阴性 (P >0 0 5 ) ;体液免疫功能测定结果为阳性 :抗体生成细胞检测 (PFC)及血清溶血素的测定结果均为阳性 (P <0 0 5或P<0 0 1) ;单核 -巨噬细胞功能测定结果为阳性 ;小鼠碳廓清实验结果为阳性 (P <0 0 1) ,小鼠腹腔巨噬细胞吞噬鸡红细胞实验为阳性 (P <0 0 5或P <0 0 1)。结论 “净常乐牌水苏糖冲剂”具有免疫调节作用
Objective To understand the immune regulation of “net Changle Stachyose Granules” on experimental mice. Methods The method was used to test the immunomodulatory effects in the Functional Food Evaluation and Testing Methods for Health Foods (Ministry of Health, 1997). Results The results showed that the cells were orally administered with 0,030 g / (kg bw) of 0,05 g / (kg bw) for 3 days, and the results of cellular immune function tests were negative. Sheep The result of DTH test in mice with SRBC was positive (P <0.01). The result of ConA - induced mouse splenic lymphocyte transformation (MTT) was negative (P> 0.05). The results of humoral immune function test were positive: the results of antibody producing cell assay (PFC) and serum hemolysin were all positive (P <0.05 or P <0.01); the results of monocyte-macrophage function test were positive (P <0.01). The mice peritoneal macrophages were positive for phagocytosis of chicken erythrocytes (P <0.05 or P <0.01). Conclusion “net Changle Stachyose Granules” has an immunomodulatory effect