论文部分内容阅读
采用常规压片法,以鹰嘴豆根尖为材料,研究了秋水仙素、对二氯苯和8-羟基喹啉等预处理剂对积累鹰嘴豆根尖细胞中期分裂相的效果,并比较了不同预处理剂对中期染色体形态的影响。结果表明,3种预处理剂均能使根尖细胞的有丝分裂停留在中期,但0.1%的秋水仙素溶液进行预处理后的中期分裂相,染色体粗短,分散度不理想;0.006 mol/L的8-羟基喹啉溶液进行预处理后的鹰嘴豆中期染色体形态不清晰,边缘缺失;对二氯苯饱和水溶液4 h处理后的根尖细胞染色体背景清晰,缢痕明显,分散度较适宜,适合核型分析。
The effect of pre-treatment agents such as colchicine, p-dichlorobenzene and 8-hydroxyquinoline on the accumulation of mid-mitotic phase in root tip cells of chickpea The effects of different pretreatment agents on metaphase chromosome morphology were compared. The results showed that all the three kinds of pretreatment agents could make mitosis of root tip cells stay in the middle stage, but 0.1% colchicine solution pretreatment of the metaphase split phase, stubble chromosomes, dispersion is not ideal; 0.006 mol / L 8-hydroxyquinoline solution pretreatment medium-sized chick pea chromosome shape is unclear, the edge of missing; 4-dichlorobenzene saturated aqueous 4 h after treatment of the root tip cell chromosome clear background, obvious marks, the dispersion is more appropriate , Suitable for karyotyping.