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An immunostimulatory factor was identified to be secreted by antigen-pulsed macrophages. This factor was able to induce the generation of antigen specific T helper lymphoeytes in vitro as well as in vivo. Further in vitro experiments testing for the genetic restriction of this factor indicated that it is a genetically restricted antigen specific factor (ASF).The Cunningham plaque assay was used to quantify the generation of T helper lymphocytes by measuring the number of plaque forming cells after sequential incubations of antigen-pulsed maerophages with T lymphoeytes, and then spleen cells, and finally the TNP-coated sheep red blood cells.
This factor was able to induce the generation of antigen specific T helper lymphoeytes in vitro as well as in vivo. Further in vitro experiments testing for the genetic restriction of this factor indicated that it is a genetically restricted antigen specific factor (ASF). The Cunningham plaque assay was used to quantify the generation of T helper lymphocytes by measuring the number of plaque forming cells after sequential incubations of antigen-pulsed maerophages with T lymphoeytes, and then spleen cells , and finally the TNP-coated sheep red blood cells.