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目的通过建立活体大鼠心肌缺血再灌注模型,模拟人类冠心病,研究聚合血红蛋白(PolyHb)在心肌缺血再灌注中的保护作用,探究PolyHb在冠心病领域中的保护和治疗作用。方法将45只Sprague-Dawley(SD)大鼠随机分成3组:实验组(15只)、对照组(15只)、假手术组(15只),建立大鼠心肌缺血模型。实验组建立动物模型后,结扎冠状动脉35min,并于结扎后5min,通过SD大鼠尾静脉按1mL/min的速度注射1mL(100g/L)的PolyHb溶液。缺血完成后开放灌注120min。对照组建立动物模型,结扎冠状动脉35min,并于结扎后5min,通过SD大鼠尾静脉按1mL/min的速度注射1mL生理盐水。缺血完成后开放灌注120min。假手术组仅建立动物模型,但不结扎冠状动脉,也不再灌注。比较3组SD大鼠的血流动力学参数左室内压最大上升和下降速率、心肌酶(血清肌酸激酶、乳酸脱氢酶)及病理学检查(梗死心肌占总心肌面积的百分比),来衡量PolyHb的作用。结果与对照组比较,用Polb处理的实验组可增强再灌注时左室内压最大上升和下降速率(P<0.05),减少血液中血清肌酸激酶和乳酸脱氢酶的含量(P<0.05),并明显减少心肌梗死面积百分比(P<0.05)。结论在心肌缺血的SD大鼠中,Polb可以有效的降低缺血再灌注损伤,从而达到心肌保护作用。
Objective To establish a model of myocardial ischemia-reperfusion in vivo and to simulate human coronary heart disease (CHD). To study the protective effect of PolyHb on myocardial ischemia-reperfusion and to explore the protective and therapeutic effects of PolyHb on coronary heart disease. Methods 45 Sprague-Dawley (SD) rats were randomly divided into three groups: experimental group (15 rats), control group (15 rats) and sham operation group (15 rats). After the animal model was established in the experimental group, the coronary arteries were ligated for 35 minutes and 5 minutes after the ligation, 1 mL (100 g / L) PolyHb solution was injected into the caudal vein of SD rats at a rate of 1 mL / min. After the completion of ischemia perfusion 120min. The control group was established animal model, coronary artery ligation 35min, and 5min after ligation, SD rat tail vein by 1mL / min speed injection of 1mL of saline. After the completion of ischemia perfusion 120min. The sham-operated group only established animal model, but did not ligate the coronary artery and did not reperfusion. The hemodynamic parameters of three groups of SD rats were compared to the maximal rise and fall rate of left ventricular pressure, myocardial enzymes (serum creatine kinase, lactate dehydrogenase) and pathological examination (the percentage of infarcted myocardium in total myocardial area) Measure PolyHb’s effect. Results Compared with the control group, the experimental group treated with Polb increased the maximum increase and decrease of left ventricular pressure at reperfusion (P <0.05), and decreased the levels of serum creatine kinase and lactate dehydrogenase (P <0.05) , And significantly reduced the myocardial infarction area percentage (P <0.05). Conclusion Polb can effectively reduce the ischemia-reperfusion injury in SD rats with myocardial ischemia and thus achieve myocardial protection.