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为研究急性心肌梗死患者外周血Ｔ淋巴细胞表达人白细胞－ＤＲ抗原的变化。将新鲜的全血或经培养的全血与抗体混合，取有核细胞，加入抗ＩｇＧ·异硫氰酸荧光素和抗ＩｇＧ·藻红蛋白，用流式细胞仪技术进行分析。结果发现：（１）ＣＤ３抗原阳性细胞无显著改变，ＣＤ８抗原阳性细胞和人白细胞－ＤＲ抗原阳性细胞显著增高，ＣＤ４抗原阳性细胞和ＣＤ４／ＣＤ８比值显著降低；（２）ＣＤ３抗原和ＤＲ抗原双阳性细胞和ＣＤ４抗原和ＤＲ抗原双阳性细胞显著增加，ＣＤ８抗原和ＤＲ抗原双阳性细胞无显著变化，ＣＤ４抗原和ＤＲ抗原与ＣＤ８抗原和ＤＲ抗原比值增高；（３）在植物凝集素、沸波醇酯和沸波醇酯＋ｉｏｎｏｍｙｃｉｎ等刺激剂存在的情况下，ＣＤ３抗原和ＤＲ抗原表达降低，ＣＤ８抗原和ＤＲ抗原表达与对照组无显著性差异。提示急性心肌梗死患者Ｔ淋巴细胞表达白细胞－ＤＲ抗原异常。
To study the changes of human leukocyte-DR antigen expression in peripheral blood T lymphocytes in patients with acute myocardial infarction. Fresh whole blood or cultured whole blood was mixed with the antibody, the nucleated cells were taken and anti-IgG, fluorescein isothiocyanate and anti-IgG phycoerythrin were added and analyzed by flow cytometry. The results showed that: (1) There was no significant change in CD3 antigen positive cells, CD8 antigen positive cells and human leukocyte-DR antigen positive cells were significantly increased, CD4 antigen positive cells and CD4 / CD8 ratio was significantly reduced; (2) CD3 antigen and DR antigen Shuangyang The number of CD4 + and DR + double positive cells was significantly increased, while there was no significant change in double positive cells of CD8 antigen and DR antigen. The ratios of CD4 antigen and DR antigen to CD8 antigen and DR antigen increased. (3) In the lectin, In the presence of stimulants such as alcohol ester and boiling alcohol ester + ionomycin, the expression of CD3 antigen and DR antigen decreased, and the expression of CD8 antigen and DR antigen showed no significant difference with the control group. Tip acute myocardial infarction in patients with T lymphocytes express leukocyte-DR antigen abnormalities.