目的对锦州市2013年流感病原学监测结果进行分析,掌握锦州地区流感的流行趋势,为流感防治工作提供科学依据。方法对采集的样本用MDCK细胞进行流感病毒分离培养,用血凝(HA)试验鉴定分离结果,用血凝抑制实验(HI)进行流感病毒型别鉴定,并且从性别、年龄、时间分布等多方面进行比较分析。结果 2013年共采集流感样病例咽拭子1 153份,进行病毒分离的样本1 153份,分离出流感病毒38株,分离率为3.30%。其中AH3亚型12株,占31.58%;新甲AH1亚型26株,占68.42%;全年流感分离阳性结果主要集中在1月、11月、12月;中年组(41岁~64岁)流感病毒分离阳性率最高为11.27%,其次是儿童组(0岁~7岁)病毒分离阳性率为6.04%。结论 2013年锦州市分离到2种流感毒株,其中新AH1型流感病毒为流行优势株。冬春季节为明显发病高峰,中老年组及儿童组是流感高发人群(χ2=64.479,P<0.05),性别差异无统计学意义(χ2=0.082,P=0.775>0.05)。 Objective To analyze the results of influenza etiology monitoring in 2013 in Jinzhou City, master the epidemiological trend of influenza in Jinzhou area and provide a scientific basis for the prevention and control of influenza. Methods The collected samples were isolated and cultured with MDCK cells. The results of the isolation were identified by the hemagglutination test (HA) and the influenza virus type by the hemagglutination inhibition test (HI). The distribution of the virus was also analyzed by gender, age and time Comparative analysis. Results In 2013, a total of 1 153 throat swabs were collected from influenza-like cases. A total of 1 153 swab samples were collected and 38 strains of influenza virus were isolated. The isolation rate was 3.30%. Among them, 12 strains of AH3 subtype accounted for 31.58%; 26 strains of AH1 subtype were identified (68.42%). The positive results of influenza isolates in the whole year mainly concentrated in January, November and December; middle-aged group (41-64 years ). The highest positive rate of influenza virus isolation was 11.27%, followed by the positive rate of virus isolation in children (0-7 years old) was 6.04%. Conclusion Two strains of influenza were isolated in Jinzhou city in 2013, of which the new type AH1 influenza virus was the predominant strain. There was no significant difference in gender between the middle-aged and elderly groups and the children group (χ2 = 64.479, P <0.05) (χ2 = 0.082, P = 0.775> 0.05).