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目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中CD4+T细胞CD25和FOXP3的表达及其临床意义。方法流式细胞术检测20例SLE患者(9例为狼疮性肾炎)及10例健康对照组PBMC中CD4+T细胞中CD25及Foxp3的表达。结果 SLE患者CD4+CD25highT细胞中FOXP3+T细胞的表达明显低于健康对照组(P<0.05),与SLE疾病活动指数SLEDAI积分明显负相关(r=-0.514,P<0.01),与补体C4正相关(r=0.362,P<0.05),与ESR及CRP负相关(r分别=-0.23、-0.216,P<0.05),与外周血免疫球蛋白IgG、IgA、IgM及补体C3水平无明显相关性。将SLE患者分为狼疮性肾炎(LN)组及非肾损(SLE-NR)组,两组CD4+CD25highT细胞及CD4+CD25lowT细胞中FOXP3+T细胞的表达均明显低于健康对照组(P<0.05);LN组CD4+T细胞中CD25high及CD25low的表达均较SLE-NR组增高(P<0.05)。结论 SLE患者外周血中CD4+CD25high T细胞中FOXP3+的表达水平明显降低,且与疾病活动性相关,而LN患者代偿增高的无FOXP3表达的CD4+CD25+T细胞可能与其发病机制有关。
Objective To investigate the expression of CD25 and FOXP3 in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE) and its clinical significance. Methods Flow cytometry was used to detect the expressions of CD25 and Foxp3 in CD4 + T cells from 20 patients with SLE (9 with lupus nephritis) and 10 healthy controls. Results The expression of FOXP3 + T cells in CD4 + CD25high T cells in SLE patients was significantly lower than that in healthy controls (P <0.05), and negatively correlated with SLE disease activity index SLEDAI scores (r = -0.514, P <0.01) (R = 0.362, P <0.05), negatively correlated with ESR and CRP (r = -0.23, -0.216, P <0.05, respectively), but no significant correlation with IgG, IgA, IgM, Correlation. The patients with SLE were divided into two groups: LN group and SLE-NR group. The expression of FOXP3 + T cells in both CD4 + CD25highT cells and CD4 + CD25lowT cells were significantly lower than those in healthy controls (P <0.05). The expressions of CD25high and CD25low in CD4 + T cells of LN group were higher than those in SLE-NR group (P <0.05). Conclusions The expression of FOXP3 + in CD4 + CD25high T cells in peripheral blood of SLE patients is significantly lower than that in patients with SLE. And the compensatory increase of CD4 + CD25 + T cells without FOXP3 in LN patients may be related to its pathogenesis.