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目的 建立沙眼衣原体 (Ct)主要外膜蛋白基因 (omp1)上游引物 5′末端地高辛标记裂解酶片段长度多态性 (CFLP)分型方法 ;了解重庆地区近年来临产孕妇Ct感染和母婴垂直传播状况及常见基因型。方法 取临产孕妇宫颈刮片及其新生儿鼻咽拭子组成母婴配对标本 30 0对 6 0 5份 ,采用Ctomp1套式聚合酶链反应 (omp1 nPCR)检测Ct;用建立的CFLP方法对临床Ct分离株进行分型。结果 孕妇宫颈Ct检出率 11% (33/ 30 0 ) ,母婴传播率 2 4 2 % (8/ 33)。生后 2 4h内和 5～ 10d采集Ct阳性母亲所生新生儿鼻咽拭子标本Ct检出率分别为 3 0 % (1/ 33)和 38 9% (7/ 18) ,χc2 =8 79,P <0 0 1。剖宫产和阴道分娩母婴传播率分别为 8 3% (2 / 2 4 )和 6 6 7% (6 / 9) ,χc2 =9 16 ,P <0 0 1。Ct阳性和阴性孕妇胎膜早破发生率分别为 30 3% (10 / 33)和 13 5 % (36 / 2 6 7) ,χ2 =6 4 0 ,P <0 0 5。 8对Ct阳性母婴配对标本CFLP图谱呈 4类 ,经测序证实分别为E、F、H、D型 (各 3、2、2、1对 ) ,各占 37 5 %、2 5 0 %、2 5 0 %和 12 5 % ,且每对母子CFLP图谱完全一致。结论 本研究结果在一定意义上反映了重庆地区近年来临产孕妇Ct感染和母婴垂直传播状况及常见基因型 ;上游引物 5′末端地高辛标记Ctomp1CFLP分型方法灵敏度
Objective To establish a method for genotyping the 5’-terminal digoxigenin-encoding fragment length polymorphism (CFLP) at the upstream of the major outer membrane protein gene (omp1) of Chlamydia trachomatis (Ct) Vertical transmission status and common genotypes. Methods 30 to 600 maternal and infant pairs of cervical smear and neonatal nasopharyngeal swab samples from pregnant women were collected for detecting Ct by Ctomp1 nested PCR (omp1 nPCR). The CFLP method Ct isolates. Results The detection rate of cervical Ct in pregnant women was 11% (33/30) and that of mother to infant was 24.2% (8/33). The Ct detection rates of nasopharyngeal swab samples from newborns born within 24 h and 5 to 10 d after birth were 30% (1/33) and 38 9% (7/18) respectively, and χc2 = 879 , P <0 0 1. Cesarean and vaginal delivery mother-infant transmission rates were 83% (2/2 4) and 6 6 7% (6/9), χc2 = 9 16, P 0 01. The incidences of premature rupture of membranes in Ct-positive and -negative pregnant women were 30 3% (13/33) and 13 5% (36/2 6 7), respectively. Eight pairs of Ct-positive maternal and infant pairs of CFLP maps were 4 categories, confirmed by sequencing were E, F, H, D type (each 3,2,2,1 pairs), each 37 5%, 25 0% 25% and 125%, respectively, and each mother and child exactly the same CFLP pattern. Conclusion The results of this study reflect Ct infection and the vertical transmission of mother and baby in Chongqing in recent years and the common genotypes of pregnant women in Chongqing. The sensitivity of the Ctomp1CFLP genotyping method of 5 ’