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油桐是我国四大木本油料植物之一,当前对油桐的研究主要集中在栽培选优及低产林改造方面,而对油桐脂肪酸延长代谢的分子机理研究尚未见报道。本研究以油桐果实膨大期、油脂转化初期和油脂转化高峰期的种子为材料,采用高通量RNA测序技术对油桐种子脂肪酸延长代谢的3个不同时期转录组进行比较,以nr、Swiss-Prot、KEGG和COG 4个蛋白数据库为参考,对油桐种子脂肪酸延长代谢进行了综合性分析。研究结果表明,参与油桐种子脂肪酸延长代谢途径的非冗余基因序列共37条,涉及的主要酶功能基因有9种;在果实膨大期、油脂转化初期和油脂转化高峰期中,调控脂肪酸延长代谢途径的基因存在明显的表达差异。综合分析结果绘制了油桐种子脂肪酸延长代谢途径,揭示了调控油桐种子脂肪酸延长代谢过程的基因作用规律。这些研究结果为油脂合成的遗传改良提供了资源和技术基础,同时为油桐分子设计育种提供了一定的科学依据。
Tung tree is one of the four major woody oil plants in our country. The current research on Tung tree mainly focuses on cultivation optimization and low-yield forest transformation. However, the molecular mechanism of Tung fatty acid prolonging metabolism has not been reported yet. In this study, the seeds of three stages of tung oilseed fatty acids lengthening metabolism were compared using high-throughput RNA sequencing technology. The results showed that nr, Swiss -Prot, KEGG and COG four protein database as a reference, a comprehensive analysis of the extended fatty acid metabolism of tung tree seed. The results showed that there were 37 non-redundant gene sequences involved in the prolonged metabolic pathway of seed fatty acid in Tung oil tree, and there were 9 major functional genes involved in them. In the period of fruit enlargement, initial oil transformation and peak oil transformation, fatty acid prolongation Pathway genes exist significant differences in expression. Based on the results of the comprehensive analysis, the fatty acids in Tung oilseeds were prolonged to extend the metabolic pathway, revealing the rules of gene regulation of fatty acids in the tung oil seedling to prolong the metabolic process. These findings provide the resources and technical basis for the genetic improvement of oil synthesis, and provide a scientific basis for the design and breeding of Tung tree molecular design.