首先将巯基DNA分子与金纳米粒子偶联,并用琼脂糖凝胶电泳分离出含不同DNA分子数目的金纳米粒子,最后将修饰有互补DNA链的Au纳米粒子进行组装,得到组装体(五聚体)。透射电子显微镜(TEM)研究表明,DNA-Au纳米组装体被成功地获得;表面增强拉曼光谱(SERS)研究表明,与未组装的金纳米粒子相比,DNA-Au纳米组装体具有更强的SERS活性。 First, mercapto-DNA molecules were conjugated with gold nanoparticles, and gold nanoparticles containing different numbers of DNA molecules were separated by agarose gel electrophoresis. Finally, Au nanoparticles modified with complementary DNA chains were assembled to obtain assemblies of pentamers body). Transmission Electron Microscopy (TEM) studies have shown that DNA-Au nanocomposites have been successfully obtained; surface-enhanced Raman spectroscopy (SERS) studies show that DNA-Au nanocomposites are more robust than unassembled gold nanoparticles SERS activity.