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目的研究骨桥蛋白(OPN)在结直肠癌中的表达,探索其与结直肠癌转移的关系。方法应用SP免疫组化技术、原位杂交技术检测60例结直肠癌(32例淋巴结转移癌,28例无淋巴结转移癌)和13例正常肠黏膜中的OPN表达。结果骨桥蛋白在80.0%(48/60)结直肠癌中、84.3%(27/32)淋巴结转移癌中和75.0%(21/28)无淋巴结淋巴结转移癌中表达,正常黏膜不表达;骨桥蛋白的表达水平同肿瘤的分化程度、淋巴结转移、Dukes分期的相关性有统计学意义(P<0.05)。骨桥蛋白mRNA在81.7%(49/60)结直肠癌、90.6%(29/32)淋巴结转移癌中表达,正常黏膜不表达;骨桥蛋白mRNA的表达水平同肿瘤的分化程度、淋巴结转移、Dukes分期的相关性有统计学意义(P<0.05)。骨桥蛋白及其mRNA在结直肠癌中的表达与正常黏膜的表达差异有统计学意义,且两者具有较好的相关性(P<0.01)。结论骨桥蛋白在结直肠癌的发生和转移可能起重要的作用,可能为结直肠癌的治疗提供新的靶点。
Objective To study the expression of osteopontin (OPN) in colorectal cancer and explore its relationship with the metastasis of colorectal cancer. Methods The expression of OPN in 60 cases of colorectal cancer (32 cases of lymph node metastasis, 28 cases of non-lymph node metastasis) and 13 cases of normal intestinal mucosa were detected by SP immunohistochemistry and in situ hybridization. RESULTS: Osteopontin was expressed in 84.0% (27/32) of lymph node metastases and 75.0% (21/28) of non-lymph node metastases in 80.0% (48/60) of colorectal carcinomas, while the normal mucosa was not expressed The expression of bridging protein was correlated with tumor differentiation, lymph node metastasis and Dukes stage (P <0.05). Osteopontin mRNA was expressed in 81.7% (49/60) of colorectal cancers and 90.6% (29/32) of lymph node metastatic cancers, but not in normal mucosa. The expression of osteopontin mRNA was correlated with tumor differentiation, lymph node metastasis, Dukes staging correlation was statistically significant (P <0.05). The expression of osteopontin and its mRNA in colorectal carcinoma was significantly different from that in normal mucosa (P <0.01). Conclusion Osteopontin may play an important role in the occurrence and metastasis of colorectal cancer and may provide a new target for the treatment of colorectal cancer.