Objective To investigate the expression of Bcl-2 and Bax in spermatogenic cellsinduced by local testicular heating in ratsMethods Forty adult male SD rats were divided into experimental group and controlgroup at random. According to day 0.5, 1, 3 and 6 after local testicular heating, eachgroup was divided into 4 subgroups: experimental subgroup (n=6) and controlsubgroup (n=4). The expression of Bcl-2 and Bax in the spermatogenic cells wasdetected on day 0.5, 1, 3 and 6 after heat exposure by using immunohistochemistry.Results Compared with control groups, the ratio of positive cells and content ofBcl-2 positive cells significantly decreased in all experimental subgroups (P<0.01).The content of Bax positive cells increased in all experimental subgroups (P<0.01),the ratio of positive cells which had no significant difference (P>0.05) except 6 dgroup decreased (P<0.01). Redistribution of Bax from a cytoplasmic to perinuclearor nuclear localization could be observed after heating.Conclusions Expression of Bcl-2 would decrease and Bax would increase with redis-tribution in spermatogenic cells in rats after heating. The change of Bcl-2 and Baxexpression in spermatogenic cells would be correlated with the spermatoge-nic cell apoptosis induced by heating. Objective To investigate the expression of Bcl-2 and Bax in spermatogenic cells induced by local testicular heating in rats Methods Forty adult male SD rats were divided into experimental group and control group at random. According to day 0.5, 1, 3 and 6 after local testicular heating, The expression of Bcl-2 and Bax in the spermatogenic cells was detected on day 0.5, 1, 3 and 6 after heat exposure by using immunohistochemistry (n = 6) and controlsubgroup .Results Compared with control groups, the ratio of positive cells and content of Bcl-2 positive cells significantly decreased in all experimental subgroups (P <0.01). The content of Bax positive cells increased in all experimental subgroups of positive cells which had no significant difference (P> 0.05) except 6 dgroup decreased (P <0.01). Redistribution of Bax from a cytoplasmic to perinuclearor nuclear could could be observed after heating. Conflicio ns Expression of Bcl-2 would decrease and Bax would increase with redis-tribution in spermatogenic cells in rats after heating. The change of Bcl-2 and Baxexpression in spermatogenic cells would be correlated with the spermatoge-nic cell apoptosis induced by heating.