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目的：研究荧光染色药敏法在急性白血病中的应用。材料和方法：用新建立的荧光染色法及甲基噻唑基四唑染色（ＭＴＴ）法检测１５例初治及复发的急性白血病病例对７种常用化疗药物的体外敏感性。结果：荧光染色及ＭＴＴ法与体内疗效的阳性符合率分别为：１００％，８６％，阴性符合率分别为：６７％，６０％；特异性分别为１００％，７５％；敏感性分别为：７８％，７５％；准确率分别为８５％，６９％。结论：荧光染色法简便，快速，经济，敏感，较ＭＴＴ法具一定优越性，对指导急性白血病的个体化治疗有较高实用价值。
Objective: To study the application of fluorescence staining drug sensitivity in acute leukemia. Materials and Methods: In vitro sensitivity of seven commonly used chemotherapeutic drugs in 15 cases of newly diagnosed and relapsed acute leukemia was detected by newly established fluorescent staining and MTT assay. RESULTS: The positive rates of fluorescence staining, MTT assay, and in vivo efficacy were: 100%, 86%. The negative coincidence rates were 67% and 60%, respectively; the specificities were 100% and 75%, respectively; the sensitivities were: 78%, 75%; accuracy rates were 85% and 69% respectively. Conclusion: The fluorescence staining method is simple, rapid, economical and sensitive. It has certain advantages over the MTT method. It has a high practical value for the individual treatment of acute leukemia.